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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP3107a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3107a, RRID:AB_2132343
- Product name
- Phospho-KDR/FLK1(Y996) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.63 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references VEGFR-1 regulates adult olfactory bulb neurogenesis and migration of neural progenitors in the rostral migratory stream in vivo.
Wittko IM, Schänzer A, Kuzmichev A, Schneider FT, Shibuya M, Raab S, Plate KH
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jul 8;29(27):8704-14
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Jul 8;29(27):8704-14
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Anti-Phospho-KDR/FLK1-Y996 Pab (Cat. #AP3107a) is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody (AP3107a)diluted to 1:500 in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
