Antibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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Validation data
Reference
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- Product number
- AF357 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal VEGFR2/KDR/Flk-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human VEGFR2/KDR/Flk-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse VEGFR2 is observed and less than 10% cross-reactivity with recombinant human (rh) VEGFR1 and rhVEGFR3 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Targeting chemoresistant colorectal cancer via systemic administration of a BMP7 variant.
ATF-2 and Tpl2 regulation of endothelial cell cycle progression and apoptosis.
Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis.
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling.
Affimer proteins are versatile and renewable affinity reagents.
VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling.
VEGF induces signalling and angiogenesis by directing VEGFR2 internalisation through macropinocytosis.
Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors.
Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
VEGF-A isoforms differentially regulate ATF-2-dependent VCAM-1 gene expression and endothelial-leukocyte interactions.
MicroRNA-24 suppression of N-deacetylase/N-sulfotransferase-1 (NDST1) reduces endothelial cell responsiveness to vascular endothelial growth factor A (VEGFA).
Essential role for endocytosis in the growth factor-stimulated activation of ERK1/2 in endothelial cells.
KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.
Differential expression of Vegfr-2 and its soluble form in preeclampsia.
Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice.
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
PEDF derived from glial Müller cells: a possible regulator of retinal angiogenesis.
VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2.
VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2.
Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Human herpesvirus 8 (HHV-8)-encoded cytokines induce expression of and autocrine signaling by vascular endothelial growth factor (VEGF) in HHV-8-infected primary-effusion lymphoma cell lines and mediate VEGF-independent antiapoptotic effects.
Veschi V, Mangiapane LR, Nicotra A, Di Franco S, Scavo E, Apuzzo T, Sardina DS, Fiori M, Benfante A, Colorito ML, Cocorullo G, Giuliante F, Cipolla C, Pistone G, Bongiorno MR, Rizzo A, Tate CM, Wu X, Rowlinson S, Stancato LF, Todaro M, De Maria R, Stassi G
Oncogene 2020 Jan;39(5):987-1003
Oncogene 2020 Jan;39(5):987-1003
ATF-2 and Tpl2 regulation of endothelial cell cycle progression and apoptosis.
Fearnley GW, Latham AM, Hollstein M, Odell AF, Ponnambalam S
Cellular signalling 2020 Feb;66:109481
Cellular signalling 2020 Feb;66:109481
Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis.
Genet G, Boyé K, Mathivet T, Ola R, Zhang F, Dubrac A, Li J, Genet N, Henrique Geraldo L, Benedetti L, Künzel S, Pibouin-Fragner L, Thomas JL, Eichmann A
Nature communications 2019 May 28;10(1):2350
Nature communications 2019 May 28;10(1):2350
Lymphatic endothelial progenitors originate from plastic myeloid cells activated by toll-like receptor-4.
Volk-Draper LD, Hall KL, Wilber AC, Ran S
PloS one 2017;12(6):e0179257
PloS one 2017;12(6):e0179257
Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling.
Jin Y, Muhl L, Burmakin M, Wang Y, Duchez AC, Betsholtz C, Arthur HM, Jakobsson L
Nature cell biology 2017 Jun;19(6):639-652
Nature cell biology 2017 Jun;19(6):639-652
Affimer proteins are versatile and renewable affinity reagents.
Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC
eLife 2017 Jun 27;6
eLife 2017 Jun 27;6
VEGFR3 Modulates Vascular Permeability by Controlling VEGF/VEGFR2 Signaling.
Heinolainen K, Karaman S, D'Amico G, Tammela T, Sormunen R, Eklund L, Alitalo K, Zarkada G
Circulation research 2017 Apr 28;120(9):1414-1425
Circulation research 2017 Apr 28;120(9):1414-1425
VEGF induces signalling and angiogenesis by directing VEGFR2 internalisation through macropinocytosis.
Basagiannis D, Zografou S, Murphy C, Fotsis T, Morbidelli L, Ziche M, Bleck C, Mercer J, Christoforidis S
Journal of cell science 2016 Nov 1;129(21):4091-4104
Journal of cell science 2016 Nov 1;129(21):4091-4104
Control of angiogenesis by galectins involves the release of platelet-derived proangiogenic factors.
Etulain J, Negrotto S, Tribulatti MV, Croci DO, Carabelli J, Campetella O, Rabinovich GA, Schattner M
PloS one 2014;9(4):e96402
PloS one 2014;9(4):e96402
Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
Shvartsman D, Storrie-White H, Lee K, Kearney C, Brudno Y, Ho N, Cezar C, McCann C, Anderson E, Koullias J, Tapia JC, Vandenburgh H, Lichtman JW, Mooney DJ
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Jul;22(7):1243-1253
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Jul;22(7):1243-1253
Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
Orecchia A, Mettouchi A, Uva P, Simon GC, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger KH, Zambruno G, Failla CM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Feb;28(2):692-704
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Feb;28(2):692-704
Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib.
Fiorio Pla A, Brossa A, Bernardini M, Genova T, Grolez G, Villers A, Leroy X, Prevarskaya N, Gkika D, Bussolati B
BMC cancer 2014 Dec 12;14:939
BMC cancer 2014 Dec 12;14:939
VEGF-A isoforms differentially regulate ATF-2-dependent VCAM-1 gene expression and endothelial-leukocyte interactions.
Fearnley GW, Odell AF, Latham AM, Mughal NA, Bruns AF, Burgoyne NJ, Homer-Vanniasinkam S, Zachary IC, Hollstein MC, Wheatcroft SB, Ponnambalam S
Molecular biology of the cell 2014 Aug 15;25(16):2509-21
Molecular biology of the cell 2014 Aug 15;25(16):2509-21
MicroRNA-24 suppression of N-deacetylase/N-sulfotransferase-1 (NDST1) reduces endothelial cell responsiveness to vascular endothelial growth factor A (VEGFA).
Kasza Z, Fredlund Fuchs P, Tamm C, Eriksson AS, O'Callaghan P, Heindryckx F, Spillmann D, Larsson E, Le Jan S, Eriksson I, Gerwins P, Kjellén L, Kreuger J
The Journal of biological chemistry 2013 Sep 6;288(36):25956-25963
The Journal of biological chemistry 2013 Sep 6;288(36):25956-25963
Essential role for endocytosis in the growth factor-stimulated activation of ERK1/2 in endothelial cells.
Gourlaouen M, Welti JC, Vasudev NS, Reynolds AR
The Journal of biological chemistry 2013 Mar 15;288(11):7467-80
The Journal of biological chemistry 2013 Mar 15;288(11):7467-80
KDR identifies a conserved human and murine hepatic progenitor and instructs early liver development.
Goldman O, Han S, Sourisseau M, Dziedzic N, Hamou W, Corneo B, D'Souza S, Sato T, Kotton DN, Bissig KD, Kalir T, Jacobs A, Evans T, Evans MJ, Gouon-Evans V
Cell stem cell 2013 Jun 6;12(6):748-60
Cell stem cell 2013 Jun 6;12(6):748-60
Differential expression of Vegfr-2 and its soluble form in preeclampsia.
Munaut C, Lorquet S, Pequeux C, Coulon C, Le Goarant J, Chantraine F, Noël A, Goffin F, Tsatsaris V, Subtil D, Foidart JM
PloS one 2012;7(3):e33475
PloS one 2012;7(3):e33475
Vascular endothelial growth factor drives autocrine epithelial cell proliferation and survival in chronic rhinosinusitis with nasal polyposis.
Lee HS, Myers A, Kim J
American journal of respiratory and critical care medicine 2009 Dec 1;180(11):1056-67
American journal of respiratory and critical care medicine 2009 Dec 1;180(11):1056-67
The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice.
Minami T, Yano K, Miura M, Kobayashi M, Suehiro J, Reid PC, Hamakubo T, Ryeom S, Aird WC, Kodama T
The Journal of clinical investigation 2009 Aug;119(8):2257-70
The Journal of clinical investigation 2009 Aug;119(8):2257-70
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
American journal of physiology. Lung cellular and molecular physiology 2006 Oct;291(4):L588-95
American journal of physiology. Lung cellular and molecular physiology 2006 Oct;291(4):L588-95
Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
The American journal of pathology 2006 Oct;169(4):1167-82
The American journal of pathology 2006 Oct;169(4):1167-82
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Günzler V, White CW
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
PEDF derived from glial Müller cells: a possible regulator of retinal angiogenesis.
Eichler W, Yafai Y, Keller T, Wiedemann P, Reichenbach A
Experimental cell research 2004 Sep 10;299(1):68-78
Experimental cell research 2004 Sep 10;299(1):68-78
VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2.
Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP
Investigative ophthalmology & visual science 2004 Feb;45(2):368-74
Investigative ophthalmology & visual science 2004 Feb;45(2):368-74
VEGF164(165) as the pathological isoform: differential leukocyte and endothelial responses through VEGFR1 and VEGFR2.
Usui T, Ishida S, Yamashiro K, Kaji Y, Poulaki V, Moore J, Moore T, Amano S, Horikawa Y, Dartt D, Golding M, Shima DT, Adamis AP
Investigative ophthalmology & visual science 2004 Feb;45(2):368-74
Investigative ophthalmology & visual science 2004 Feb;45(2):368-74
Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
International journal of cancer 2003 May 1;104(5):603-10
International journal of cancer 2003 May 1;104(5):603-10
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Bataille R, Almici C, Caramatti C, Mangoni L, Rizzoli V
Blood 2003 Jul 15;102(2):638-45
Blood 2003 Jul 15;102(2):638-45
Human herpesvirus 8 (HHV-8)-encoded cytokines induce expression of and autocrine signaling by vascular endothelial growth factor (VEGF) in HHV-8-infected primary-effusion lymphoma cell lines and mediate VEGF-independent antiapoptotic effects.
Liu C, Okruzhnov Y, Li H, Nicholas J
Journal of virology 2001 Nov;75(22):10933-40
Journal of virology 2001 Nov;75(22):10933-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human VEGF R2/KDR/Flk-1 by Western Blot. Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human VEGF R2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for VEGF R2/KDR/Flk-1 at approximately 200-250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- VEGF R2/KDR/Flk-1 in Human Kidney. VEGF R2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human VEGF R2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- VEGF R2/KDR/Flk-1 in Human Placenta. VEGF R2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 15 µg/mL Goat Anti-Human VEGF R2/ KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- VEGF R2/KDR/Flk-1 Inhibi-tion of VEGF-dependent Cell Proliferation and Neutral-ization by Human VEGF R2/KDR/Flk-1 Anti-body. Recombi-nant Human VEGF R2/KDR/Flk-1 Fc Chi-mera (Catalog # 357-KD) inhibits Recombinant Human VEGF165 (Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF165 (5 ng/mL) activity elicited by Recombinant Human VEGF R2/KDR/Flk-1 Fc Chi-mera (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF R2/KDR/Flk-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF357). The ND50 is typically 0.05-0.25 µg/mL.