DM3502

antibody from Acris Antibodies GmbH

Targeting: KDR CD309, FLK1, VEGFR, VEGFR2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM3502

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#DM3502, RRID:AB_1008479

Product name

anti CD309 / VEGFR-2 / Flk-1

Antibody type

Monoclonal

Antigen

Recombinant Human soluble extracellular KDR (D7) (110 kDa) (Cat.-No DA3530)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

KDREIC

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Analysis of anti-Human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Functional ELISA with anti-human VEGFR-2/KDR Cat.-No DM3502. Recombinant human soluble KDR (D7) Cat.-No DA3530X was coated with increasing amounts in a 96 well microtiter plate. The antibody was used at 1μg/ml.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis of VEGFR-2/KDR expression in HUVE cells (upper level) and EPCs derived from PBMcs (lower level ) (5μg/ml) Cat.-No DM3502 C, 5μg/ml PE goat anti-Mouse IgG.The experiment was performed by Trisha M. Westerhof, University of California, Irvine.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

FACS analysis of VEGFR-2/KDR expression in HUVE cells.