Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB357F-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VEGFR2/KDR/Flk-1 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human VEGF R2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGF R1, rhVEGF R3 or recombinant mouse VEGF R2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P35968
- Isotype
- IgG
- Antibody clone number
- 89106
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Leucocyte and Platelet-rich Fibrin: a carrier of autologous multipotent cells for regenerative medicine.
Engineered ligand-based VEGFR antagonists with increased receptor binding affinity more effectively inhibit angiogenesis.
Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction.
Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments.
Heterochromatin Protein 1 Alpha (HP1α: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells.
Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells.
Di Liddo R, Bertalot T, Borean A, Pirola I, Argentoni A, Schrenk S, Cenzi C, Capelli S, Conconi MT, Parnigotto PP
Journal of cellular and molecular medicine 2018 Mar;22(3):1840-1854
Journal of cellular and molecular medicine 2018 Mar;22(3):1840-1854
Engineered ligand-based VEGFR antagonists with increased receptor binding affinity more effectively inhibit angiogenesis.
Kapur S, Silverman AP, Ye AZ, Papo N, Jindal D, Blumenkranz MS, Cochran JR
Bioengineering & translational medicine 2017 Mar;2(1):81-91
Bioengineering & translational medicine 2017 Mar;2(1):81-91
Innovative Flow Cytometry Allows Accurate Identification of Rare Circulating Cells Involved in Endothelial Dysfunction.
Boraldi F, Bartolomeo A, De Biasi S, Orlando S, Costa S, Cossarizza A, Quaglino D
PloS one 2016;11(8):e0160153
PloS one 2016;11(8):e0160153
Levels of circulating endothelial cells are low in idiopathic pulmonary fibrosis and are further reduced by anti-fibrotic treatments.
De Biasi S, Cerri S, Bianchini E, Gibellini L, Persiani E, Montanari G, Luppi F, Carbonelli CM, Zucchi L, Bocchino M, Zamparelli AS, Vancheri C, Sgalla G, Richeldi L, Cossarizza A
BMC medicine 2015 Nov 9;13:277
BMC medicine 2015 Nov 9;13:277
Heterochromatin Protein 1 Alpha (HP1α: CBX5) is a Key Regulator in Differentiation of Endothelial Progenitor Cells to Endothelial Cells.
Maeng YS, Kwon JY, Kim EK, Kwon YG
Stem cells (Dayton, Ohio) 2015 May;33(5):1512-22
Stem cells (Dayton, Ohio) 2015 May;33(5):1512-22
Expression of soluble vascular endothelial growth factor receptor-1 in human monocyte-derived mature dendritic cells contributes to their antiangiogenic property.
Kishuku M, Nishioka Y, Abe S, Kishi J, Ogino H, Aono Y, Azuma M, Kinoshita K, Batmunkh R, Makino H, Ranjan P, Minakuchi K, Sone S
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8176-85
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):8176-85
Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells.
Xiong YQ, Sun HC, Zhang W, Zhu XD, Zhuang PY, Zhang JB, Wang L, Wu WZ, Qin LX, Tang ZY
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Aug 1;15(15):4838-46
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Aug 1;15(15):4838-46
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of VEGF R2/KDR/Flk-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGF R2/KDR/Flk-1 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB357F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.