MAB3572-100

antibody from R&D Systems

Targeting: KDR CD309, FLK1, VEGFR, VEGFR2

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3572-100

Provider

R&D Systems

Product name

Human VEGFR2/KDR/Flk-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human VEGFR2/KDR/Flk-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) VEGFR1, rhVEGFR3 or recombinant mouse VEGFR2 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P35968


Isotype

IgG

Antibody clone number

89106

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Bone marrow characterization in COPD: a multi-level network analysis.

Toledo-Pons N, Noell G, Jahn A, Iglesias A, Duran MA, Iglesias J, Rios A, Scrimini S, Faner R, Gigirey O, Agustí A, Cosío BG
Respiratory research 2018 Jun 15;19(1):118

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Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells.

Odent Grigorescu G, Rosca AM, Preda MB, Tutuianu R, Simionescu M, Burlacu A
Journal of tissue engineering and regenerative medicine 2017 Nov;11(11):3241-3252

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Identification of a Novel Pathogenic Germline KDR Variant in Melanoma.

Silva IP, Salhi A, Giles KM, Vogelsang M, Han SW, Ismaili N, Lui KP, Robinson EM, Wilson MA, Shapiro RL, Pavlick A, Zhong J, Kirchhoff T, Osman I
Clinical cancer research : an official journal of the American Association for Cancer Research 2016 May 15;22(10):2377-85

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Vasoreparative dysfunction of CD34+ cells in diabetic individuals involves hypoxic desensitization and impaired autocrine/paracrine mechanisms.

Jarajapu YP, Hazra S, Segal M, Li Calzi S, Jadhao C, Qian K, Mitter SK, Raizada MK, Boulton ME, Grant MB
PloS one 2014;9(4):e93965

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MicroRNA-16 affects key functions of human endothelial progenitor cells.

Goretti E, Rolland-Turner M, Léonard F, Zhang L, Wagner DR, Devaux Y
Journal of leukocyte biology 2013 May;93(5):645-55

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Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.

Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
Nature 2011 Jul 13;475(7355):226-30

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Vascular endothelial growth factor as an autocrine survival factor for retinal pigment epithelial cells under oxidative stress via the VEGF-R2/PI3K/Akt.

Byeon SH, Lee SC, Choi SH, Lee HK, Lee JH, Chu YK, Kwon OW
Investigative ophthalmology & visual science 2010 Feb;51(2):1190-7

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Resistance of acute myeloid leukemic cells to the triterpenoid CDDO-Imidazolide is associated with low caspase-8 and FADD levels.

Riccioni R, Senese M, Diverio D, Riti V, Mariani G, Boe A, LoCoco F, Foà R, Peschle C, Sporn M, Testa U
Leukemia research 2008 Aug;32(8):1244-58

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Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.

Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
Biochemical and biophysical research communications 2008 Apr 11;368(3):543-9

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Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.

Ball SG, Shuttleworth CA, Kielty CM
The Journal of cell biology 2007 May 7;177(3):489-500

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Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.

Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
Stem cells (Dayton, Ohio) 2007 Feb;25(2):490-9

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Dissecting the role of endothelial SURVIVIN DeltaEx3 in angiogenesis.

Caldas H, Fangusaro JR, Boué DR, Holloway MP, Altura RA
Blood 2007 Feb 15;109(4):1479-89

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Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.

Lin YL, Liang YC, Chiang BL
Journal of leukocyte biology 2007 Dec;82(6):1473-80

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Expression of Tie-2 and other receptors for endothelial growth factors in acute myeloid leukemias is associated with monocytic features of leukemic blasts.

Riccioni R, Diverio D, Mariani G, Buffolino S, Riti V, Saulle E, Petrucci E, Cedrone M, Lo-Coco F, Foà R, Peschle C, Testa U
Stem cells (Dayton, Ohio) 2007 Aug;25(8):1862-71

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Thiol regulation of vascular endothelial growth factor-A and its receptors in human retinal pigment epithelial cells.

Sreekumar PG, Kannan R, de Silva AT, Burton R, Ryan SJ, Hinton DR
Biochemical and biophysical research communications 2006 Aug 11;346(4):1200-6

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of VEGFR2/KDR/Flk-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3572, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

VEGFR2/KDR Inhibition of VEGF-dependent Cell Proliferation and Neutralization by Human VEGFR2/KDR Antibody. Recombinant Human VEGFR2/KDR Fc Chimera (Catalog # 357-KD) inhibits Recombinant Human VEGF₁₆₅ (Catalog # 293-VE) induced proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Inhibition of Recombinant Human VEGF₁₆₅ (10 ng/mL) activity elicited by Recombinant Human VEGFR2/KDR Fc Chimera (50 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human VEGFR2/KDR Monoclonal Antibody (Catalog # MAB3572). The ND₅₀ is typically 10-50 ng/mL.