Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB4432 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse VEGFR2/KDR/Flk-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse VEGFR2/KDR/Flk-1.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P35918
- Isotype
- IgG
- Antibody clone number
- 522302
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references M2-polarized macrophages contribute to neovasculogenesis, leading to relapse of oral cancer following radiation.
Okubo M, Kioi M, Nakashima H, Sugiura K, Mitsudo K, Aoki I, Taniguchi H, Tohnai I
Scientific reports 2016 Jun 8;6:27548
Scientific reports 2016 Jun 8;6:27548
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VEGFR2/KDR/Flk-1 in bEnd.3 Mouse Cell Line. VEGFR2/KDR/Flk-1 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB4432) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of VEGFR2/KDR/Flk-1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endo-thelioma cell line was stained with Rat Anti-Mouse VEGFR2/KDR/Flk-1 Mono-clonal Antibody (Catalog # MAB4432, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113).