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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB0505 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0505, RRID:AB_983010
- Product name
- KDR (phospho Y996) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of KDR.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of vascular endothelial growth factor receptor 2-mediated phosphorylation of focal adhesion kinase by heat shock protein 90 and Src kinase activities.
Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms.
VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia.
Le Boeuf F, Houle F, Huot J
The Journal of biological chemistry 2004 Sep 10;279(37):39175-85
The Journal of biological chemistry 2004 Sep 10;279(37):39175-85
Platelet factor 4 disrupts the intracellular signalling cascade induced by vascular endothelial growth factor by both KDR dependent and independent mechanisms.
Sulpice E, Contreres JO, Lacour J, Bryckaert M, Tobelem G
European journal of biochemistry / FEBS 2004 Aug;271(16):3310-8
European journal of biochemistry / FEBS 2004 Aug;271(16):3310-8
VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia.
Lee YK, Bone ND, Strege AK, Shanafelt TD, Jelinek DF, Kay NE
Blood 2004 Aug 1;104(3):788-94
Blood 2004 Aug 1;104(3):788-94
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) is used in Western blot to detect Phospho-KDR-Y996 in HMVEC cell lysate. Endothelial cells were stimulated with 50 ug/mL VEGF for 5min; 20 ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) diluted in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
