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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB12647 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12647, RRID:AB_10552511
- Product name
- KDR polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of KDR.
- Storage
- Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Analysis of protein-protein interactions in cross-talk pathways reveals CRKL protein as a novel prognostic marker in hepatocellular carcinoma.
Soluble vascular endothelial growth factor receptor 3 is essential for corneal alymphaticity.
VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis.
Liu CH, Chen TC, Chau GY, Jan YH, Chen CH, Hsu CN, Lin KT, Juang YL, Lu PJ, Cheng HC, Chen MH, Chang CF, Ting YS, Kao CY, Hsiao M, Huang CY
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49
Molecular & cellular proteomics : MCP 2013 May;12(5):1335-49
Soluble vascular endothelial growth factor receptor 3 is essential for corneal alymphaticity.
Singh N, Tiem M, Watkins R, Cho YK, Wang Y, Olsen T, Uehara H, Mamalis C, Luo L, Oakey Z, Ambati BK
Blood 2013 May 16;121(20):4242-9
Blood 2013 May 16;121(20):4242-9
VEGF receptor-2 Y951 signaling and a role for the adapter molecule TSAd in tumor angiogenesis.
Matsumoto T, Bohman S, Dixelius J, Berge T, Dimberg A, Magnusson P, Wang L, Wikner C, Qi JH, Wernstedt C, Wu J, Bruheim S, Mugishima H, Mukhopadhyay D, Spurkland A, Claesson-Welsh L
The EMBO journal 2005 Jul 6;24(13):2342-53
The EMBO journal 2005 Jul 6;24(13):2342-53
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The whole cell lysate derived from human umbilical vein endothelial cell (HUVEC) was stimulated by VEGF-A for 20 min, then immunoprecipitated by KDR polyclonal antibody (Cat # PAB12647) followed by immune-probing with KDR polyclonal antibody (Cat # PAB12647) at 1 : 500. The immunoreactive bands are observed around ~ 200 KDa (non-phospho form) and ~ 240 KDa (Phospho-form, lane 1). The lane 2 is a negative control.
- Validation comment
- Immunoprecipitation
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human breast cancer tissue was stained with KDR polyclonal antibody (Cat # PAB12647) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
