RQ4055

antibody from NSJ Bioreagents

Targeting: KDR CD309, FLK1, VEGFR, VEGFR2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4055

Provider

NSJ Bioreagents

Product name

Vegf receptor 2 Antibody / Vegfr2

Antibody type

Polyclonal

Description

This highly specific Vegf receptor 2 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry applications with mouse and rat samples.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Vegf receptor 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of recombinant mouse Vegfr2 protein with Vegf receptor 2 antibody at 0.5ug/ml.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of recombinant mouse Vegfr2 protein with Vegf receptor 2 antibody at 0.5ug/ml.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse 1) heart, 2) thymus, 3) spleen, 4) kidney, 5) brain, 6) lung, 7) HEPA1-6 and 8) NIH 3T3 lysate with Vegf receptor 2 antibody. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human NIH 3T3 cells with Vegf receptor 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE rat kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MCF7 cells with Vegf receptor 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Vegf receptor 2 antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human LLC1 cells with Vegf receptor 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Vegf receptor 2 antibody.