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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NB100-627 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-627, RRID:AB_10001875
- Product name
- Rabbit Polyclonal VEGFR2/KDR/Flk-1 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Sheep
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Hydrogen sulfide suppresses endoplasmic reticulum stress-induced endothelial-to-mesenchymal transition through Src pathway.
Hypoxia-Inducible Factor-1α Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model.
Impaired expression of HIF-2α induces compensatory expression of HIF-1α for the recovery from anemia.
Expression of periostin in human breast cancer.
The carboxyl terminus of VEGFR-2 is required for PKC-mediated down-regulation.
The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling.
Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells.
Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity.
Ying R, Wang XQ, Yang Y, Gu ZJ, Mai JT, Qiu Q, Chen YX, Wang JF
Life sciences 2016 Jan 1;144:208-17
Life sciences 2016 Jan 1;144:208-17
Hypoxia-Inducible Factor-1α Is Associated With Sprouting Angiogenesis in the Murine Laser-Induced Choroidal Neovascularization Model.
André H, Tunik S, Aronsson M, Kvanta A
Investigative ophthalmology & visual science 2015 Oct;56(11):6591-604
Investigative ophthalmology & visual science 2015 Oct;56(11):6591-604
Impaired expression of HIF-2α induces compensatory expression of HIF-1α for the recovery from anemia.
Tsuboi I, Yamashita T, Nagano M, Kimura K, To'a Salazar G, Ohneda O
Journal of cellular physiology 2015 Jul;230(7):1534-48
Journal of cellular physiology 2015 Jul;230(7):1534-48
Expression of periostin in human breast cancer.
Puglisi F, Puppin C, Pegolo E, Andreetta C, Pascoletti G, D'Aurizio F, Pandolfi M, Fasola G, Piga A, Damante G, Di Loreto C
Journal of clinical pathology 2008 Apr;61(4):494-8
Journal of clinical pathology 2008 Apr;61(4):494-8
The carboxyl terminus of VEGFR-2 is required for PKC-mediated down-regulation.
Singh AJ, Meyer RD, Band H, Rahimi N
Molecular biology of the cell 2005 Apr;16(4):2106-18
Molecular biology of the cell 2005 Apr;16(4):2106-18
The carboxyl terminus controls ligand-dependent activation of VEGFR-2 and its signaling.
Meyer RD, Singh AJ, Rahimi N
The Journal of biological chemistry 2004 Jan 2;279(1):735-42
The Journal of biological chemistry 2004 Jan 2;279(1):735-42
Receptor chimeras indicate that the vascular endothelial growth factor receptor-1 (VEGFR-1) modulates mitogenic activity of VEGFR-2 in endothelial cells.
Rahimi N, Dayanir V, Lashkari K
The Journal of biological chemistry 2000 Jun 2;275(22):16986-92
The Journal of biological chemistry 2000 Jun 2;275(22):16986-92
Vascular endothelial growth factor and hepatocyte growth factor levels are differentially elevated in patients with advanced retinopathy of prematurity.
Lashkari K, Hirose T, Yazdany J, McMeel JW, Kazlauskas A, Rahimi N
The American journal of pathology 2000 Apr;156(4):1337-44
The American journal of pathology 2000 Apr;156(4):1337-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Detection of VEGFR2 in CSF-VEGF R2/KDR/Flk-1 transfected lysates using NB 100-627. ECL detection in 15 seconds. Lane 1 - 1:2,000 Lane 2 - 1:10,000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Analysis of VEGF R2/KDR/Flk-1 in mouse brain tumor lysates using anti-VEGF R2/KDR/Flk-1 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Lane 1 is 20 ug, lane 2 is 40 ug and lane 3 is 50 ug of protein. KDR primary antibody was used at 1:500. The secondary was HRP goat anti-rabbit at 1:4,000. ECL detection was at 86 sec. This image was submitted via customer Review. *{Sheep WB}*
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody [NB100-627] - Human placenta using VEGF R2 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Syncitiotrophoblast staining was observed. Staining was performed by Histowiz.
