Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Flow cytometry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB200-208 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-208, RRID:AB_10000504
- Product name
- Mouse Monoclonal VEGFR2/KDR/Flk-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The monoclonal will detect native human VEGFR-2/ KDR in ELISA experiments and on the surface of different human cell types.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Stromal cell-derived factor-1alpha activation of tissue-engineered endothelial progenitor cell matrix enhances ventricular function after myocardial infarction by inducing neovasculogenesis.
Acute myocardial rescue with endogenous endothelial progenitor cell therapy.
Frederick JR, Fitzpatrick JR 3rd, McCormick RC, Harris DA, Kim AY, Muenzer JR, Marotta N, Smith MJ, Cohen JE, Hiesinger W, Atluri P, Woo YJ
Circulation 2010 Sep 14;122(11 Suppl):S107-17
Circulation 2010 Sep 14;122(11 Suppl):S107-17
Acute myocardial rescue with endogenous endothelial progenitor cell therapy.
Atluri P, Panlilio CM, Liao GP, Hiesinger W, Harris DA, McCormick RC, Cohen JE, Jin T, Feng W, Levit RD, Dong N, Woo YJ
Heart, lung & circulation 2010 Nov;19(11):644-54
Heart, lung & circulation 2010 Nov;19(11):644-54
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis using the Biotin conjugate of NB200-208. Detection of anti-human VEGFR-2/KDR. Samples were loaded in 10% SDS-polyacrylamide gel under reducing conditions.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Functional ELISA with anti-human VEGFR-2/KDR. Recombinant human soluble KDR (D7) was coated with increasing amounts in a 96 well microtiter plate. The antibody was used at 1ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis of VEGFR-2/KDR expression in HUVE cells (upper level) and EPCs derived from PBMcs (lower level ) [5ug/ml]; 5ug/ml PE goat anti-mouse IgG]. Image provided by Trisha M. Westerhof, University of California, Irvine.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Analysis using the Biotin conjugate of NB200-208. Staining of VEGFR-2/KDR expression in HUVE cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [NB200-208] - Flow Cytometry: VEGF R2/KDR/Flk-1 Antibody (EIC) [Allophycocyanin] [NB200-208APC] - HUVECs were unstained (left) or stained (right) with VEGFR-2/KDR. Image from verified customer review. Image using the Allophycocyanin form of this antibody.