12332-1-AP

antibody from Proteintech Group

Targeting: IDH1

Provider product page for 12332-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [72]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

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Product number: 12332-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12332-1-AP, RRID:AB_2123159
Product name: IDH1 antibody
Antibody type: Polyclonal
Description: KD/KO validated IDH1 antibody (Cat. #12332-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IDH1 protein structure - 12332-1-AP shown in red.

Submitted references Mitochondrial inhibition enhances the sensitivity of pancreatic ductal adenocarcinoma cells to oncolytic adenovirus.

Shoji R, Tazawa H, Kuroda S, Nishiyama T, Kajiwara Y, Yamada M, Nagai Y, Inoue H, Hashimoto N, Kikuchi S, Yoshida R, Umeda Y, Urata Y, Kagawa S, Fujiwara T
Molecular therapy. Oncology 2026 Jun 18;34(2):201180

Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy.

Zhang Y, Shen X, Shen Y, Wang C, Yu C, Han J, Cao S, Qian L, Ma M, Huang S, Wen W, Yin M, Lei QY
Nature 2026 Jan;649(8098):1022-1031

Glutamine supports human cytomegalovirus induced lipidome remodeling through reductive carboxylation.

Mokry RL, de Lacy CB, Purdy JG
bioRxiv : the preprint server for biology 2026 Jan 27;

Lactate derived from macrophages drives skin dermal fibroblasts phenotypic remodeling via MCT1-primed histone H3 lysine 23 lactylation in hypertrophic scar.

Yuan Y, Xiao Y, Zou J, Luo L, Li M, Shen K, Wei L, Zhang Y, Wang P, Chen Y, Zhuo S, Zhang H, Song S, Jia Y, Wang K, Jiang S, Guan H, Hu D
Nature communications 2026 Feb 12;17(1)

Convallatoxin ameliorates fibroblast-like synoviocytes-mediated synovial inflammation and joint destruction in rheumatoid arthritis by targeting IDH1.

Liu S, Hu H, Kuang Y, Chen S, Sun K, Peng C, Li R, Su F, Liang L, Qiu Q, Xiao Y, Xu H
Naunyn-Schmiedeberg's archives of pharmacology 2026 Apr;399(7):10579-10597

WTAP Maintains Alternative Activation of Macrophages by Promoting IDH1-Mediated α-ketoglutarate Production.

Xu Q, Zhang J, Zou Y, Chen L, Sun F, Luo X, Wang T, Li Y, Zhang S, Xiong F, Yu Q, Yang P, Gong Q, Liu SW, Wang CY
International journal of biological sciences 2025;21(12):5428-5443

Application of the Mass Spectrometry-High-Throughput Technique Over the Immunohistochemical Analysis for Human Brain Tumor Diagnosis and Prognosis: Insights Into Biomarkers' Identification for the Case Study of Grade IV Astrocytomas and Meningiomas.

Louati K, Kolsi F, Mellouli M, Louati H, Kallel R, Zribi R, Borni M, Hakim LS, Maalej A, Choura S, Chamkha M, Sayadi S, Khemakhem Z, Boudawara TS, Boudawara MZ, Zribi K, Safta F
Biomedical chromatography : BMC 2025 Sep;39(9):e70170

Metabolic immune regulation of macrophages by melanized fungus Fonsecaea monophora.

Pan Q, Xin T, Zhang J, Lin L, Zhang J
Journal de mycologie medicale 2025 Sep;35(3):101571

The Identification by Shotgun Proteomics with High-Resolution Tandem Mass-Spectrometry of Histone Isoforms' Hypermethylation Phenotype as a Hallmark Characteristic of Human-IDH-Mutant High-Grade Gliomas: Epigenetic Applications for Genotoxicity-Based Biomarkers and Cancer Therapy Targets.

Louati K, Kolsi F, Mellouli M, Louati H, Zribi R, Kallel R, Borni M, Gdoura Y, Hakim LS, Maalej A, Choura S, Chamkha M, Sayadi S, Mnif B, Khemakhem Z, Boudawara TS, Boudawara MZ, Bouraoui A, Kraiem J, Safta F
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A galacturonan from sea buckthorn (Hippophae rhamnoides L.) berry modulates dyslipidemia by promoting hepatic lipolysis and mitochondrial oxidation.

Sun Y, Ni W, Wang D, Ren J, Zhao D, Yu S, Liu S, Zhang S, Wang S, Wang W, Liu M
Carbohydrate polymers 2025 Sep 1;363:123708

SIRT7 drives energy metabolic shifts in endometriosis via interaction with TUFM and Rhoa/Rock/Akt pathway activation.

Zhang H, Chen J, Yang M, Ding X, Chai G, Huang R, Lin D, Huang Z, Chen Q
Cellular and molecular life sciences : CMLS 2025 Oct 30;82(1):381

CHIT1 regulates the neuroinflammation and phagocytosis of microglia and suppresses Aβ plaque deposition in Alzheimer's disease.

Yuan S, Zhong F, Wan T, Qin Z, Chen L, Xing D, Zhang W, Yu W, Huang L, Song J, Yu W, Lü Y
The Journal of pathology 2025 Mar;265(3):330-341

Identifying the target, mechanism, and agonist of α-ketoglutaric acid in delaying mesenchymal stem cell senescence.

Cui Z, Li J, Li C, Deng S, Liu W, Lei T, Cao J, Wang Z, Wang X, Ma S, Zhu Y, Yang H, Chen P
Cell reports 2025 Jul 22;44(7):115917

Metformin Induces Apoptosis and Ferroptosis of Ovarian Cancer Cells Under Energy Stress Conditions.

Wu Y, Zhang Z, Ren M, Chen Y, Zhang J, Li J, Gao F, Bao Y, Huang Y, Yang X, Song Z
Cells 2025 Feb 2;14(3)

Kaempferol exerts anti-colorectal cancer effects through its multi-target mediated glucose metabolism remodeling.

Wu H, Guo Z, Hu P, Du J, Wang P, Ma J, Zhao B, Wei J, Li Z
Food & function 2025 Dec 8;16(24):9593-9608

IDH1 regulates human erythropoiesis by eliciting chromatin state reprogramming.

Li M, Zhang H, Wu X, Yu M, Yang Q, Sun L, Li W, Jiang Z, Xue F, Wang T, An X, Chen L
eLife 2025 Apr 29;13

A novel biomarker GATM suppresses proliferation and malignancy of cholangiocarcinoma cells by modulating the JNK/c-Jun signalling pathways.

Yu Y, Gan W, Xiong J, Li J
Heliyon 2024 Sep 15;10(17):e37344

Gaining Insight into the Catalytic Mechanism of the R132H IDH1 Mutant: A Synergistic DFT Cluster and Experimental Investigation.

Broome JA, Nguyen NP, Baumung CRE, Chen VC, Bushnell EAC
Biochemistry 2024 Oct 15;63(20):2682-2691

Acrylamide Exposure Impairs Ovarian Tricarboxylic Acid Cycle and Reduces Oocyte Quality in Mouse.

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Environmental toxicology 2024 Nov;39(11):5074-5085

Acrolein exposure affects ovarian function by interfering with glycolysis and mitochondrial energy metabolism in mouse.

Chen YZ, Wang WK, Yang YF, Cheng SY, Li LF, Shen H, Qi ZM, Liu Y
Environmental pollution (Barking, Essex : 1987) 2024 Nov 15;361:124776

Loss of the mitochondrial carrier, SLC25A1, during embryogenesis induces a unique senescence program controlled by p53.

Kasprzyk-Pawelec A, Tan M, Rahhal R, McIntosh A, Fernandez H, Mosaoa R, Jiang L, Pearson GW, Glasgow E, Vockley J, Albanese C, Avantaggiati ML
bioRxiv : the preprint server for biology 2024 May 31;

The acetylation of MDH1 and IDH1 is associated with energy metabolism in acute liver failure.

Shi C, Zhang Y, Chen Q, Wang Y, Zhang D, Guo J, Zhang Q, Zhang W, Gong Z
iScience 2024 May 17;27(5):109678

Metabolic Bypass Rescues Aberrant S-nitrosylation-Induced TCA Cycle Inhibition and Synapse Loss in Alzheimer's Disease Human Neurons.

Andreyev AY, Yang H, Doulias PT, Dolatabadi N, Zhang X, Luevanos M, Blanco M, Baal C, Putra I, Nakamura T, Ischiropoulos H, Tannenbaum SR, Lipton SA
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Mar;11(12):e2306469

RIOK3 sustains colorectal cancer cell survival under glucose deprivation via an HSP90α-dependent pathway.

Zhang N, Dong L, Ning T, Du F, Zhao M, Xu J, Xie S, Liu S, Sun X, Li P, Zhang S, Zhu S
Oncogenesis 2024 Mar 7;13(1):12

Deacetylated MDH1 and IDH1 aggravates PANoptosis in acute liver failure through endoplasmic reticulum stress signaling.

Shi C, Wang Y, Guo J, Zhang D, Zhang Y, Gong Z
Cell death discovery 2024 Jun 8;10(1):275

Effects of protoscoleces excretory-secretory products of Echinococcus granulosus on hepatocyte growth, function, and glucose metabolism.

Luo G, Li H, Lu Q, Cao J, Lv H, Jiang Y
Acta tropica 2024 Jan;249:107066

IDH1/MDH1 deacetylation promotes acute liver failure by regulating NETosis.

Wang Y, Shi C, Guo J, Zhang D, Zhang Y, Zhang L, Gong Z
Cellular & molecular biology letters 2024 Jan 3;29(1):8

Real-time monitoring of glucose metabolism and effects of metformin on HepG2 cells using (13)C in-cell NMR spectroscopy.

Teng M, Li Z, Gu Y, Fan Y, Wang D, Liu M, Li Y, Wei G, Huang Y
Biochemical and biophysical research communications 2024 Jan 29;694:149383

Scutellarin activates IDH1 to exert antitumor effects in hepatocellular carcinoma progression.

Cui Z, Li C, Liu W, Sun M, Deng S, Cao J, Yang H, Chen P
Cell death & disease 2024 Apr 15;15(4):267

Ovarian juvenile granulosa cell tumors with Ollier's disease in children with IDH1 gene somatic mutation.

Zhang J, Hua R, Ma L, Liu C, Zhang Y, Lü X, Wang T, Wan N
Frontiers in endocrinology 2023;14:1093273

Extracellular ATP-induced calcium oscillations regulating the differentiation of osteoblasts through aerobic oxidation metabolism pathways.

Gao X, Di X, Li J, Kang Y, Xie W, Sun L, Zhang J
Journal of bone and mineral metabolism 2023 Sep;41(5):606-620

HDAC6 deacetylates IDH1 to promote the homeostasis of hematopoietic stem and progenitor cells.

Yang J, Liu Y, Yin H, Xie S, Zhang L, Dong X, Ni H, Bu W, Ma H, Liu P, Zhu H, Guo R, Sun L, Wu Y, Qin J, Sun B, Li D, Luo HR, Liu M, Xuan C, Zhou J
EMBO reports 2023 Oct 9;24(10):e56009

Lycorine promotes IDH1 acetylation to induce mitochondrial dynamics imbalance in colorectal cancer cells.

Zhuo FF, Li L, Liu TT, Liang XM, Yang Z, Zheng YZ, Luo QW, Lu JH, Liu D, Zeng KW, Tu PF
Cancer letters 2023 Oct 1;573:216364

Differential Proteome Profiling Analysis under Pesticide Stress by the Use of a Nano-UHPLC-MS/MS Untargeted Proteomic-Based Approach on a 3D-Developed Neurospheroid Model: Identification of Protein Interactions, Prognostic Biomarkers, and Potential Therapeutic Targets in Human IDH Mutant High-Grade Gliomas.

Louati K, Maalej A, Kolsi F, Kallel R, Gdoura Y, Borni M, Hakim LS, Zribi R, Choura S, Sayadi S, Chamkha M, Mnif B, Khemakhem Z, Boudawara TS, Boudawara MZ, Safta F
Journal of proteome research 2023 Nov 3;22(11):3534-3558

Extracellular Calcium-Induced Calcium Transient Regulating the Proliferation of Osteoblasts through Glycolysis Metabolism Pathways.

Gao X, Di X, Li J, Kang Y, Xie W, Sun L, Zhang J
International journal of molecular sciences 2023 Mar 5;24(5)

TNFα-induced IDH1 hyperacetylation reprograms redox homeostasis and promotes the chemotherapeutic sensitivity.

Yang H, Zhao X, Liu J, Jin M, Liu X, Yan J, Yao X, Mao X, Li N, Liang B, Xie W, Zhang K, Zhao J, Liu L, Huang G
Oncogene 2023 Jan;42(1):35-48

Disabling Uncompetitive Inhibition of Oncogenic IDH Mutations Drives Acquired Resistance.

Lyu J, Liu Y, Gong L, Chen M, Madanat YF, Zhang Y, Cai F, Gu Z, Cao H, Kaphle P, Kim YJ, Kalkan FN, Stephens H, Dickerson KE, Ni M, Chen W, Patel P, Mims AS, Borate U, Burd A, Cai SF, Yin CC, You MJ, Chung SS, Collins RH, DeBerardinis RJ, Liu X, Xu J
Cancer discovery 2023 Jan 9;13(1):170-193

Huangqi-Danshen decoction reshapes renal glucose metabolism profiles that delays chronic kidney disease progression.

Huang X, Gao L, Deng R, Peng Y, Wu S, Lu J, Liu X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023 Aug;164:114989

Malonylation of Acetyl-CoA carboxylase 1 promotes hepatic steatosis and is attenuated by ketogenic diet in NAFLD.

Cao H, Cai Q, Guo W, Su Q, Qin H, Wang T, Xian Y, Zeng L, Cai M, Guan H, Chen S, Liang H, Xu F
Cell reports 2023 Apr 25;42(4):112319

IDH1 Promotes Foam Cell Formation by Aggravating Macrophage Ferroptosis.

Li B, Wang C, Lu P, Ji Y, Wang X, Liu C, Lu X, Xu X, Wang X
Biology 2022 Sep 23;11(10)

Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.

Wu Y, Chen K, Li L, Hao Z, Wang T, Liu Y, Xing G, Liu Z, Li H, Yuan H, Lu J, Zhang C, Zhang J, Zhao D, Wang J, Nie J, Ye D, Pan G, Chan WY, Liu X
Cell death and differentiation 2022 Nov;29(11):2316-2331

Therapeutic targeting of glutamate dehydrogenase 1 that links metabolic reprogramming and Snail-mediated epithelial-mesenchymal transition in drug-resistant lung cancer.

Wang Q, Wu M, Li H, Rao X, Ao L, Wang H, Yao L, Wang X, Hong X, Wang J, Aa J, Sun M, Wang G, Liu J, Zhou F
Pharmacological research 2022 Nov;185:106490

Grossamide attenuates inflammation by balancing macrophage polarization through metabolic reprogramming of macrophages in mice.

Zhao W, Xu D, Hong W, Zhang L, Wu Q, Gao M, Wang J
International immunopharmacology 2022 Nov;112:109190

Anti-inflammation of torachrysone-8-O-β-ᴅ-glucoside by hurdling over morphological changes of macrophages.

Zhao W, Xu D, Zhang L, Meng H, Zheng Q, Wang J
International immunopharmacology 2022 Apr;105:108548

Wild-type IDH1 inhibits the tumor growth through degrading HIF-α in renal cell carcinoma.

Chen S, Wang Y, Xiong Y, Peng T, Lu M, Zhang L, Guo Z
International journal of biological sciences 2021;17(5):1250-1262

Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer Biology.

Errington TM, Denis A, Allison AB, Araiza R, Aza-Blanc P, Bower LR, Campos J, Chu H, Denson S, Donham C, Harr K, Haven B, Iorns E, Kwok J, McDonald E, Pelech S, Perfito N, Pike A, Sampey D, Settles M, Scott DA, Sharma V, Tolentino T, Trinh A, Tsui R, Willis B, Wood J, Young L
eLife 2021 Dec 7;10

Withanolides from dietary tomatillo suppress HT1080 cancer cell growth by targeting mutant IDH1.

Yang Y, Xiang K, Sun D, Zheng M, Song Z, Li M, Wang X, Li H, Chen L
Bioorganic & medicinal chemistry 2021 Apr 15;36:116095

Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling.

Chen X, Zhuo S, Xu W, Chen X, Huang D, Sun X, Cheng Y
British journal of cancer 2020 Jul;123(1):126-136

α-Ketoglutarate links p53 to cell fate during tumour suppression.

Morris JP 4th, Yashinskie JJ, Koche R, Chandwani R, Tian S, Chen CC, Baslan T, Marinkovic ZS, Sánchez-Rivera FJ, Leach SD, Carmona-Fontaine C, Thompson CB, Finley LWS, Lowe SW
Nature 2019 Sep;573(7775):595-599

Insight into novel RNA-binding activities via large-scale analysis of lncRNA-bound proteome and IDH1-bound transcriptome.

Liu L, Li T, Song G, He Q, Yin Y, Lu JY, Bi X, Wang K, Luo S, Chen YS, Yang Y, Sun BF, Yang YG, Wu J, Zhu H, Shen X
Nucleic acids research 2019 Mar 18;47(5):2244-2262

Metabolic reprogramming and Notch activity distinguish between non-small cell lung cancer subtypes.

Sellers K, Allen TD, Bousamra M 2nd, Tan J, Méndez-Lucas A, Lin W, Bah N, Chernyavskaya Y, MacRae JI, Higashi RM, Lane AN, Fan TW, Yuneva MO
British journal of cancer 2019 Jul;121(1):51-64

Loss of IDH2 Accelerates Age-related Hearing Loss in Male Mice.

White K, Kim MJ, Han C, Park HJ, Ding D, Boyd K, Walker L, Linser P, Meneses Z, Slade C, Hirst J, Santostefano K, Terada N, Miyakawa T, Tanokura M, Salvi R, Someya S
Scientific reports 2018 Mar 22;8(1):5039

LncRNA IDH1-AS1 links the functions of c-Myc and HIF1α via IDH1 to regulate the Warburg effect.

Xiang S, Gu H, Jin L, Thorne RF, Zhang XD, Wu M
Proceedings of the National Academy of Sciences of the United States of America 2018 Feb 13;115(7):E1465-E1474

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.

Intlekofer AM, Wang B, Liu H, Shah H, Carmona-Fontaine C, Rustenburg AS, Salah S, Gunner MR, Chodera JD, Cross JR, Thompson CB
Nature chemical biology 2017 May;13(5):494-500

G6pd Deficiency Does Not Affect the Cytosolic Glutathione or Thioredoxin Antioxidant Defense in Mouse Cochlea.

White K, Kim MJ, Ding D, Han C, Park HJ, Meneses Z, Tanokura M, Linser P, Salvi R, Someya S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Jun 7;37(23):5770-5781

HIF-1α inhibits IDH-1 expression in osteosarcoma.

Liu DC, Zheng X, Zho Y, Yi WR, Li ZH, Hu X, Yu AX
Oncology reports 2017 Jul;38(1):336-342

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Zhu Z, Li C, Zeng Y, Ding J, Qu Z, Gu J, Ge L, Tang F, Huang X, Zhou C, Wang P, Zheng D, Jin Y
Cell stem cell 2017 Feb 2;20(2):274-289.e7

Curcumol inhibits the proliferation of gastric adenocarcinoma MGC-803 cells via downregulation of IDH1.

Zang S, Tang Q, Dong F, Liu H, Li L, Guo F, Pan X, Lin H, Zeng W, Cai Z, Zhong Q, Zang N, Zang L
Oncology reports 2017 Dec;38(6):3583-3591

Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage.

Liu L, Shah S, Fan J, Park JO, Wellen KE, Rabinowitz JD
Nature chemical biology 2016 May;12(5):345-52

Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation.

Richarson AD, Scott DA, Zagnitko O, Aza-Blanc P, Chang CC, Russler-Germain DA, Reproducibility Project: Cancer Biology
eLife 2016 Mar 11;5:e10860

Loss of 5-hydroxymethylcytosine is linked to gene body hypermethylation in kidney cancer.

Chen K, Zhang J, Guo Z, Ma Q, Xu Z, Zhou Y, Xu Z, Li Z, Liu Y, Ye X, Li X, Yuan B, Ke Y, He C, Zhou L, Liu J, Ci W
Cell research 2016 Jan;26(1):103-18

Prognostic and diagnostic potential of isocitrate dehydrogenase 1 in esophageal squamous cell carcinoma.

Chen X, Li Q, Wang C, Xu W, Han L, Liu Y, Liu B, Guan S, Tan B, Wang J, Wang N, Song Q, Jia Y, Wang J, Zhao L, Cheng Y
Oncotarget 2016 Dec 27;7(52):86148-86160

Proteomic profile in Perna viridis after exposed to Prorocentrum lima, a dinoflagellate producing DSP toxins.

Huang L, Zou Y, Weng HW, Li HY, Liu JS, Yang WD
Environmental pollution (Barking, Essex : 1987) 2015 Jan;196:350-7

Quantitative flux analysis reveals folate-dependent NADPH production.

Fan J, Ye J, Kamphorst JJ, Shlomi T, Thompson CB, Rabinowitz JD
Nature 2014 Jun 12;510(7504):298-302

Up-regulated isocitrate dehydrogenase 1 suppresses proliferation, migration and invasion in osteosarcoma: in vitro and in vivo.

Hu X, Liu Y, Qin C, Pan Z, Luo J, Yu A, Cheng Z
Cancer letters 2014 Apr 28;346(1):114-21

Proteomic investigation of the sinulariolide-treated melanoma cells A375: effects on the cell apoptosis through mitochondrial-related pathway and activation of caspase cascade.

Li HH, Su JH, Chiu CC, Lin JJ, Yang ZY, Hwang WI, Chen YK, Lo YH, Wu YJ
Marine drugs 2013 Jul 22;11(7):2625-42

Proteomic analysis of the differences in orbital protein expression in thyroid orbitopathy.

Cheng KC, Huang HH, Hung CT, Chen CC, Wu WC, Suen JL, Chen KJ, Wu YJ, Chang CH
Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 2013 Dec;251(12):2777-87

IDH mutation impairs histone demethylation and results in a block to cell differentiation.

Lu C, Ward PS, Kapoor GS, Rohle D, Turcan S, Abdel-Wahab O, Edwards CR, Khanin R, Figueroa ME, Melnick A, Wellen KE, O'Rourke DM, Berger SL, Chan TA, Levine RL, Mellinghoff IK, Thompson CB
Nature 2012 Feb 15;483(7390):474-8

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Su CC, Su JH, Lin JJ, Chen CC, Hwang WI, Huang HH, Wu YJ
Marine drugs 2011 Dec;9(12):2622-2642

Liver X receptor agonists augment human islet function through activation of anaplerotic pathways and glycerolipid/free fatty acid cycling.

Ogihara T, Chuang JC, Vestermark GL, Garmey JC, Ketchum RJ, Huang X, Brayman KL, Thorner MO, Repa JJ, Mirmira RG, Evans-Molina C
The Journal of biological chemistry 2010 Feb 19;285(8):5392-404

Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.

Figueroa ME, Abdel-Wahab O, Lu C, Ward PS, Patel J, Shih A, Li Y, Bhagwat N, Vasanthakumar A, Fernandez HF, Tallman MS, Sun Z, Wolniak K, Peeters JK, Liu W, Choe SE, Fantin VR, Paietta E, Löwenberg B, Licht JD, Godley LA, Delwel R, Valk PJ, Thompson CB, Levine RL, Melnick A
Cancer cell 2010 Dec 14;18(6):553-67

Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.

Dang L, White DW, Gross S, Bennett BD, Bittinger MA, Driggers EM, Fantin VR, Jang HG, Jin S, Keenan MC, Marks KM, Prins RM, Ward PS, Yen KE, Liau LM, Rabinowitz JD, Cantley LC, Thompson CB, Vander Heiden MG, Su SM
Nature 2009 Dec 10;462(7274):739-44

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: MCF7 cells were subjected to SDS PAGE followed by western blot with 12332-1-AP(IDH1 antibody) at dilution of 1:600
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The IDH1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human IDH1. This antibody recognizes human,mouse,rat antigen. The IDH1 antibody has been validated for the following applications: ELISA, IHC, IP, IF, WB analysis.
Appendix: 12332-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-IDH1 (IP:12332-1-AP, 3ug; Detection:12332-1-AP 1:600) with MCF-7 cells lysate 2500ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human kidney using 12332-1-AP(IDH1 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue