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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA035248 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA035248, RRID:AB_10600714
- Product name
- Anti-IDH1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human IDH1, Gene description: isocitrate dehydrogenase 1 (NADP+), soluble, Validated applications: IHC, WB, Uniprot ID: O75874, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Clinicopathological correlation of glioma patients with respect to immunohistochemistry markers: A prospective study of 115 patients in a Tertiary Care Hospital in North India
Identification of a novel inactivating mutation in Isocitrate Dehydrogenase 1 (IDH1-R314C) in a high grade astrocytoma
The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis
Dahuja G, Gupta A, Jindal A, Jain G, Sharma S, Kumar A
Asian Journal of Neurosurgery 2022;16(04):732-737
Asian Journal of Neurosurgery 2022;16(04):732-737
Identification of a novel inactivating mutation in Isocitrate Dehydrogenase 1 (IDH1-R314C) in a high grade astrocytoma
van Lith S, Navis A, Lenting K, Verrijp K, Schepens J, Hendriks W, Schubert N, Venselaar H, Wevers R, van Rooij A, Wesseling P, Molenaar R, van Noorden C, Pusch S, Tops B, Leenders W
Scientific Reports 2016;6(1)
Scientific Reports 2016;6(1)
The Proteomics of Colorectal Cancer: Identification of a Protein Signature Associated with Prognosis
Addison C, O'Dwyer D, Ralton L, O'Shea A, Murray G
PLoS ONE 2011;6(11):e27718
PLoS ONE 2011;6(11):e27718
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-IDH1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human prostate and skeletal muscle tissues using HPA035248 antibody. Corresponding IDH1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
