Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-46018 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Isocitrate Dehydrogenase 1/IDH1 Antibody
- Antibody type
- Monoclonal
- Description
- Affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - Analysis of HEK293T cells were either not tranfected (left lane "293T") or transfected with pCMV6-ENTRY IDH1 (wild type-SKU# RC210582, middle lane "WT") or pCMV6-ENTRY IDH1 mutated (R132H mutation-SKU# RC400096, right lane "R132H") cDNA for 48 hrs and lysed.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - Analysis of extracts (35ug) from 9 different cell lines. HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY IDH1 (RC210582, right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - HEK293T cells were either not tranfected (left lane "293T") or transfected with pCMV6-ENTRY IDH1 (wild type, middle lane "WT") or pCMV6-ENTRY IDH1 mutated (R132H mutation, right lane "R132H") cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with NBP2-46018 (1:500) and then goat anti-mouse IgG-HRP (1:2000).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - Immunohistochemical staining of paraffin-embedded human endometrium tissue within the normal limits using anti-IDH1 mouse monoclonal antibody. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100C for 10 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (OTI3G9) [NBP2-46018] - Immunohistochemical staining of paraffin-embedded human lymphoma tissue using anti-IDH1 mouse monoclonal antibody. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100C for 10 min.