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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-31599 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-31599, RRID:AB_2123168
- Product name
- Rabbit Polyclonal Isocitrate Dehydrogenase 1/IDH1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate dehydrogenase Antibody [NBP1-31599] - Sample (30 ug of whole cell lysate) A: A431 B: HepG2 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate dehydrogenase Antibody [NBP1-31599] - A431 (Lane A) and Raji (Lane B), with 10% SDS PAGE, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate dehydrogenase Antibody [NBP1-31599] - Sample (30 ug of whole cell lysate) A: JC B: C2C12 10% SDS PAGE; antibody diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - A. 30 ug Neuro2A whole cell extract B. 30 ug C8D30 whole cell extract C. 30 ug NIH-3T3 whole cell extract D. 30 ug Raw 264.7 whole cell extract E. 30 ug C2Cl2 whole cell extract 10 % SDS-PAGE IDH1 antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with IDH1 antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 ug of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate dehydrogenase Antibody [NBP1-31599] - Paraffin-embedded colon ca, using antibody at 1:100 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Rat colon. IDH1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Mouse duodenum. IDH1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
