51-2400

antibody from Invitrogen Antibodies

Targeting: PTEN BZS, MHAM, MMAC1, PTEN1, TEP1

ELISA

Antibody data

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Validation data

PTEN protein structure - 51-2400 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human prostate tissue stained with rabbit anti-PTEN antibody (Product # 18-0256).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of PTEN showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PTEN polyclonal antibody (Product # 51-2400) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of PTEN showing staining in the cytoplasm of paraffin-embedded human prostate carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PTEN polyclonal antibody (Product # 51-2400) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of PTEN showing staining in the cytoplasm and nucleus of paraffin-embedded mouse prostate tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PTEN polyclonal antibody (Product # 51-2400) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Melatonin increased the miR-21-5p targets Spry1 and PTEN expression, but not Smurf2 or PDCD4. The levels of Spry1 (A), PTEN (B), Smurf2 (C) and PDCD4 (D) protein expression were examined by Western blot inNRK-49F cells co-treated with TGF-beta1 (2 ng/mL) and 10 umol/L of melatonin for 48 h; n = 6, * P < .01, & P < .05 compared with control group, # P < .01, SS P < .05, compared with TGF-beta1 + Vehicle group. The level of alpha-SMA protein in NRK-49F cells was evaluated by Western blot. NRK-49F cells were transfected with Spry1 siRNA/control siRNA (E) and PTEN siRNA/control siRNA (F), respectively, and then co-cultured with TGF-beta1 (2 ng/mL) with or without 10 umol/L of melatonin for 48 h; n = 6, * P < .01 compared with control group, # P < .01 compared with TGF-beta1 + Vehicle group, P < .01 compared with TGF-beta1 + melatonin + control siRNA

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 MiR-21-5p alleviated renoprotective effects of melatonin and inhibited up-regulation of Spry1 and PTEN induced by melatonin in the kidney tissues of unilateral ureteral obstruction (UUO) mice. A, Renal tubular injury was assessed by haematoxylin and eosin (HE) staining (x200). Scale bar = 50 um. B, The deposition of collagen in kidney tissues of UUO rats was determined by Masson''s trichrome staining (x200). Blue (aniline blue) represents collagen fibres; red (acid fuchsin) represents muscle fibres. Scale bar = 50 um. C and D, The statistical analyses of tubulointerstitial damage scores and the degrees of interstitial collagen deposition in different groups. E, RT-PCR analysis of the expression of miR-21-5p in kidneys of mice in different group. F, Western blot analysis of the expression of PTEN and Spry1 in different groups. Bars represent means +- standard deviation (SD) (n = 8). * P < .01 vs the Sham group; # P < .05, SS P < .01 vs the UUO group; Delta P < 0.05, PS P < .01 vs the UUO + Mel + agomir group; Omega P < 0.05, |= P < 0.01 vs the UUO + Mel group. Mel, melatonin; NC, control agomir

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Level of PTEN, PI3K, and mTOR protein expression according to breast cancer subtype. IHC, Immunohistochemistry.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Western blotting to detect PTEN overexpression and Akt phosphorylation in NIH3T3 cells. ( A ) Confirmation of PTEN overexpression in transfected NIH3T3 cells; the empty vector control is shown. ( B ) Akt phosphorylation in NIH3T3 cells. Cells were serum-starved for 24 h before induction of Akt phosphorylation via a brief 5 min treatment with 2 ng/mL EGF, before harvesting protein lysates. ( C ) Densitometric quantification of Akt phosphorylation in ( B ), shown as the ratio of phosphorylated to total Akt. EGF = epithelial growth factor; pTT = pTarget vector; WT = wild-type.