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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX130418 - Provider product page
- Provider
- GeneTex
- Product name
- PTEN (phospho Thr366/Ser370) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) A431 whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/pS370) antibody (GTX130418) diluted at 1:500. (CIP: Calf-intestinal alkaline phosphatase)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PTEN (phospho Thr366/Ser370) antibody detects PTEN (phospho Thr366/Ser370) protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PTEN (phospho Thr366/Ser370) protein stained by PTEN (phospho Thr366/Ser370) antibody (GTX130418) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
