Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- IC847G-100UG - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat PTEN Alexa Fluor® 488-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human, mouse, and rat PTEN.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
P60484
- Isotype
- IgG
- Antibody clone number
- 217702
- Vial size
- 100 ug
- Concentration
- 0.2 mg/ml
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references VprBP (DCAF1) Regulates RAG1 Expression Independently of Dicer by Mediating RAG1 Degradation.
Schabla NM, Perry GA, Palmer VL, Swanson PC
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):930-939
Journal of immunology (Baltimore, Md. : 1950) 2018 Aug 1;201(3):930-939
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of PTEN in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell lymphocytes were stained with Mouse Anti-Human/Mouse/Rat PTEN Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC847G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.