PAB12724

antibody from Abnova Corporation

Targeting: PTEN BZS, MHAM, MMAC1, PTEN1, TEP1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12724

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12724, RRID:AB_10556485

Product name

PTEN polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of PTEN.

Storage

Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.

Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T
The Journal of biological chemistry 2005 Oct 21;280(42):35195-202

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12724) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate tissue stained with PTEN polyclonal antibody (Cat # PAB12724) at 1 : 100 for 10 min at RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)