PAB12725

antibody from Abnova Corporation

Targeting: PTEN BZS, MHAM, MMAC1, PTEN1, TEP1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12725

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12725, RRID:AB_10554392

Product name

PTEN polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of PTEN.

Storage

Store at 4°C. For long term storage store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Cooperative phosphorylation of the tumor suppressor phosphatase and tensin homologue (PTEN) by casein kinases and glycogen synthase kinase 3beta.

Al-Khouri AM, Ma Y, Togo SH, Williams S, Mustelin T
The Journal of biological chemistry 2005 Oct 21;280(42):35195-202

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

The mouse brain tissue lysate resolved onto 10% SDS-PAGE, transferred onto NC membrane, and followed by an immunoblotting with PTEN polyclonal antibody (Cat # PAB12725) (Lane 1) at 1 : 500, or with a pre-incubation of immunizing peptide (Lane 2).

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot Blot : 1 ug peptide was blot onto NC membrane.A : PTEN (pS385) (Phosphospecific) B : PTEN (Paired S385) (Non phosphospeicfic) C : Non-related phosphospecific peptidewere blotted at a 1 : 2000 dilution by :1 : Phospho-PTEN S385 polyclonal antibody (Cat # PAB12751),2 : PTEN polyclonal antibody (Cat # PAB12725).

Validation comment

Dot Blot (Peptide)