MA5-15560

antibody from Invitrogen Antibodies

Targeting: PTEN BZS, MHAM, MMAC1, PTEN1, TEP1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15560

Provider

Invitrogen Antibodies

Product name

PTEN Monoclonal Antibody (1B8)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15560 targets PTEN in indirect ELISA, ICC, IF, FACS, and WB applications and shows reactivity with Human and mouse samples. The MA5-15560 immunogen is purified recombinant fragment of PTEN expressed in E. Coli. MA5-15560 detects PTEN which has a predicted molecular weight of approximately 54kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1B8

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTEN using PTEN monoclonal antibody (Product # MA5-15560) in HeLa (1) and NIH/3T3 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PTEN using PTEN monoclonal antibody (Product # MA5-15560) in HeLa (1) and NIH/3T3 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa (left) and HepG2 (right) cells using PTEN monoclonal antibody (Product # MA5-15560) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using PTEN monoclonal antibody (Product # MA5-15560) (right) and negative control (left).