Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
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Validation data
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- Product number
- PA5-20418 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTEN Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is P815 cell lysate. PA5-20418 can be used with blocking peptide PEP-0535.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references RedoxiFluor: A microplate technique to quantify target-specific protein thiol redox state in relative percentage and molar terms.
Tuncay A, Noble A, Guille M, Cobley JN
Free radical biology & medicine 2022 Mar;181:118-129
Free radical biology & medicine 2022 Mar;181:118-129
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of P815 cell lysate using a PTEN polyclonal antibody (Product # PA5-20418) at (A) 1 and (B) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PTEN in human brain tissue lysate with PTEN Polyclonal Antibody (Product # PA5-20418) at (A) 1 and (B) 2 µg/mL..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of P815 cells using a PTEN polyclonal antibody (Product # PA5-20418) at a 2 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of PTEN in P815 cells with PTEN Polyclonal Antibody (Product # PA5-20418) at 2 µg/mL.