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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- NBP2-22177 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal eIF4E Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - Western blot analysis using eIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4E Antibody (5D11) [NBP2-22177] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eIF4E (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eIF4E.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: eIF4E Antibody (5D11) [NBP2-22177] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Staining of paraffin-embedded Human pancreas tissue using anti-eIF4E mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Staining of paraffin-embedded Human Kidney tissue using anti-eIF4E mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-eIF4E mouse monoclonal antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using eIF4E mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: eIF4E Antibody (5D11) [NBP2-22177] - Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using eIF4E mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: eIF4E Antibody (5D11) [NBP2-22177] - Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).
