Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AM1852B - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1852b, RRID:AB_10815776
- Product name
- EIF4E Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- EIF4E Antibody (Cat. #AM1852b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).
- Primary Ab dilution
- 1:100~500
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent image of Hela cell stained with EIF4E Antibody(Cat#AM1852b/SG101020AF).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). EIF4E immunoreactivity is localized to Cytoplasm significantly."
- Primary Ab dilution
- 1:10~50