AM1852B

antibody from Abgent

Targeting: EIF4E EIF4E1, EIF4EL1, EIF4F

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM1852B

Provider

Abgent

Proper citation

Abgent Cat#AM1852b, RRID:AB_10815776

Product name

EIF4E Antibody

Antibody type

Monoclonal

Antigen

Recombinant Protein

Description

Mouse Monoclonal Antibody (Mab)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

400 µl

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

EIF4E Antibody (Cat. #AM1852b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).

Primary Ab dilution

1:100~500

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Fluorescent image of Hela cell stained with EIF4E Antibody(Cat#AM1852b/SG101020AF).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!). EIF4E immunoreactivity is localized to Cytoplasm significantly."

Primary Ab dilution

1:10~50