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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AM1852a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1852a, RRID:AB_10814010
- Product name
- EIF4E Antibody (Ascites)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- EIF4E Antibody (Cat. #AM1852a) western blot analysis in mouse NIH-3T3 cell line lysates (15ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- EIF4E Antibody (Cat. #AM1852a) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
