Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX132092 - Provider product page
- Provider
- GeneTex
- Product name
- eIF4E antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Inhibition of WNT-CTNNB1 signaling upregulates SQSTM1 and sensitizes glioblastoma cells to autophagy blockers.
Nàger M, Sallán MC, Visa A, Pushparaj C, Santacana M, Macià A, Yeramian A, Cantí C, Herreros J
Autophagy 2018;14(4):619-636
Autophagy 2018;14(4):619-636
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 293T whole cell and cytoplasm extracts (30 £gg) were separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with eIF4E antibody (GTX132092) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eIF4E antibody detects eIF4E protein at cytoplasm by immunofluorescent analysis.Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: eIF4E protein stained by eIF4E antibody (GTX132092) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- eIF4E antibody detects EIF4E protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. eIF4E antibody (GTX132092) diluted at 1:500.
