Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Other assay [1]
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Validation data
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- Product number
- PAB8147 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8147, RRID:AB_1707789
- Product name
- EIF4E (phospho S209) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of EIF4E.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Assignment of two of the translation initiation factor-4E (EIF4EL1 and EIF4EL2) genes to human chromosomes 4 and 20.
Chromosomal assignment of one of the mammalian translation initiation factor eIF-4E genes.
Phosphorylation site of eukaryotic initiation factor 4E.
Pelletier J, Brook JD, Housman DE
Genomics 1991 Aug;10(4):1079-82
Genomics 1991 Aug;10(4):1079-82
Chromosomal assignment of one of the mammalian translation initiation factor eIF-4E genes.
Dorfman J, Lazaris-Karatzas A, Malo D, Sonenberg N, Gros P
Genomics 1991 Apr;9(4):785-8
Genomics 1991 Apr;9(4):785-8
Phosphorylation site of eukaryotic initiation factor 4E.
Rychlik W, Russ MA, Rhoads RE
The Journal of biological chemistry 1987 Aug 5;262(22):10434-7
The Journal of biological chemistry 1987 Aug 5;262(22):10434-7
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Dot blot analysis of EIF4E (phospho S209) polyclonal antibody (Cat # PAB8147) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)