TA502993

antibody from OriGene

Targeting: EIF4E EIF4E1, EIF4EL1, EIF4F

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502993

Provider

OriGene

Proper citation

OriGene Cat#TA502993, RRID:AB_11124179

Product name

EIF4E mouse monoclonal antibody, clone OTI6C11 (formerly 6C11)

Antibody type

Monoclonal

Description

EIF4E mouse monoclonal antibody, clone OTI6C11 (formerly 6C11)

Host

Mouse

Conjugate

Unconjugated

Epitope

EIF4E

Isotype

IgG

Antibody clone number

OTI6C11

Vial size

100 µl

Concentration

0.39 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-EIF4E monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)

Validation comment

WB

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-EIF4E antibody(TA502993),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC