V9192

antibody from NSJ Bioreagents

Targeting: EIF4E EIF4E1, EIF4EL1, EIF4F

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V9192

Provider

NSJ Bioreagents

Product name

Eukaryotic translation initiation factor 4E Antibody / EIF4E

Antibody type

Monoclonal

Description

This highly specific Eukaryotic translation initiation factor 4E antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

PCRP-EIF4E-1D3

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the Eukaryotic translation initiation factor 4E antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of PFA-fixed human MCF-7 cells using Eukaryotic translation initiation factor 4E antibody (green, clone PCRP-EIF4E-1D3) and phalloidin (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of PFA-fixed human HeLa cells using Eukaryotic translation initiation factor 4E antibody (green, clone PCRP-EIF4E-1D3).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining of PFA-fixed human HeLa cells with Eukaryotic translation initiation factor 4E antibody (blue, clone PCRP-EIF4E-1D3) and isotype control (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining of PFA-fixed human Raji cells with Eukaryotic translation initiation factor 4E antibody (blue, clone PCRP-EIF4E-1D3), and isotype control (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining of PFA-fixed human U-87 cells with Eukaryotic translation initiation factor 4E antibody (blue, clone PCRP-EIF4E-1D3), and isotype control (red).

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Eukaryotic translation initiation factor 4E antibody (clone PCRP-EIF4E-1D3). These results demonstrate the foremost specificity of the PCRP-EIF4E-1D3 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Eukaryotic translation initiation factor 4E antibody (clone PCRP-EIF4E-1D3) as confirmation of integrity and purity.