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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Western blot [2]
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- Product number
- NB100-58833 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-58833, RRID:AB_877748
- Product name
- Rabbit Polyclonal eIF4E Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references CUL3 Deficiency Causes Social Deficits and Anxiety-like Behaviors by Impairing Excitation-Inhibition Balance through the Promotion of Cap-Dependent Translation.
Dong Z, Chen W, Chen C, Wang H, Cui W, Tan Z, Robinson H, Gao N, Luo B, Zhang L, Zhao K, Xiong WC, Mei L
Neuron 2020 Feb 5;105(3):475-490.e6
Neuron 2020 Feb 5;105(3):475-490.e6
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4E Antibody [NB100-58833] - Detection of Human and Mouse eIF4E on HeLa whole cell lysate using NB100-58833. eIF4E was also immunoprecipitated by rabbit anti-eIF4E antibody NB100-58832.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4E Antibody [NB100-58833] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-eIF4E antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
