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- Antibody Data
- Antigen structure
- References [27]
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- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF1042 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal PDGF R beta Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 35% cross-reactivity with recombinant human PDGF R beta .
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Glioblastoma Exhibits Inter-Individual Heterogeneity of TSPO and LAT1 Expression in Neoplastic and Parenchymal Cells.
GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury.
The isolation and molecular characterization of cerebral microvessels.
Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes.
Exercise promotes a cardioprotective gene program in resident cardiac fibroblasts.
TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung.
Straightforward method for singularized and region-specific CNS microvessels isolation.
Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-β42 by LRP1-dependent apolipoprotein E isoform-specific mechanism.
NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy.
PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma.
Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2.
Ageing causes prominent neurovascular dysfunction associated with loss of astrocytic contacts and gliosis.
Disruption of Bmal1 Impairs Blood-Brain Barrier Integrity via Pericyte Dysfunction.
Dual effects of carbon monoxide on pericytes and neurogenesis in traumatic brain injury.
Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.
Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation.
Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke.
Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
Nogo receptor blockade overcomes remyelination failure after white matter stroke and stimulates functional recovery in aged mice.
Restoration of oligodendrocyte pools in a mouse model of chronic cerebral hypoperfusion.
Malignant stroma increases luminal breast cancer cell proliferation and angiogenesis through platelet-derived growth factor signaling.
Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility.
Pericyte loss influences Alzheimer-like neurodegeneration in mice.
Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice.
Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
Cai L, Kirchleitner SV, Zhao D, Li M, Tonn JC, Glass R, Kälin RE
International journal of molecular sciences 2020 Jan 17;21(2)
International journal of molecular sciences 2020 Jan 17;21(2)
GPR124 facilitates pericyte polarization and migration by regulating the formation of filopodia during ischemic injury.
Chen DY, Sun NH, Lu YP, Hong LJ, Cui TT, Wang CK, Chen XH, Wang SS, Feng LL, Shi WX, Fukunaga K, Chen Z, Lu YM, Han F
Theranostics 2019;9(20):5937-5955
Theranostics 2019;9(20):5937-5955
The isolation and molecular characterization of cerebral microvessels.
Lee YK, Uchida H, Smith H, Ito A, Sanchez T
Nature protocols 2019 Nov;14(11):3059-3081
Nature protocols 2019 Nov;14(11):3059-3081
Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes.
Diéguez-Hurtado R, Kato K, Giaimo BD, Nieminen-Kelhä M, Arf H, Ferrante F, Bartkuhn M, Zimmermann T, Bixel MG, Eilken HM, Adams S, Borggrefe T, Vajkoczy P, Adams RH
Nature communications 2019 Jun 27;10(1):2817
Nature communications 2019 Jun 27;10(1):2817
Exercise promotes a cardioprotective gene program in resident cardiac fibroblasts.
Lighthouse JK, Burke RM, Velasquez LS, Dirkx RA Jr, Aiezza A 2nd, Moravec CS, Alexis JD, Rosenberg A, Small EM
JCI insight 2019 Jan 10;4(1)
JCI insight 2019 Jan 10;4(1)
TBX2-positive cells represent a multi-potent mesenchymal progenitor pool in the developing lung.
Wojahn I, Lüdtke TH, Christoffels VM, Trowe MO, Kispert A
Respiratory research 2019 Dec 23;20(1):292
Respiratory research 2019 Dec 23;20(1):292
Straightforward method for singularized and region-specific CNS microvessels isolation.
Dayton JR, Franke MC, Yuan Y, Cruz-Orengo L
Journal of neuroscience methods 2019 Apr 15;318:17-33
Journal of neuroscience methods 2019 Apr 15;318:17-33
Blood-brain barrier-associated pericytes internalize and clear aggregated amyloid-β42 by LRP1-dependent apolipoprotein E isoform-specific mechanism.
Ma Q, Zhao Z, Sagare AP, Wu Y, Wang M, Owens NC, Verghese PB, Herz J, Holtzman DM, Zlokovic BV
Molecular neurodegeneration 2018 Oct 19;13(1):57
Molecular neurodegeneration 2018 Oct 19;13(1):57
NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy.
Dubrac A, Künzel SE, Künzel SH, Li J, Chandran RR, Martin K, Greif DM, Adams RH, Eichmann A
Nature communications 2018 Aug 27;9(1):3463
Nature communications 2018 Aug 27;9(1):3463
PDGF-mediated mesenchymal transformation renders endothelial resistance to anti-VEGF treatment in glioblastoma.
Liu T, Ma W, Xu H, Huang M, Zhang D, He Z, Zhang L, Brem S, O'Rourke DM, Gong Y, Mou Y, Zhang Z, Fan Y
Nature communications 2018 Aug 27;9(1):3439
Nature communications 2018 Aug 27;9(1):3439
Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2.
Tsutsumi R, Harizanova J, Stockert R, Schröder K, Bastiaens PIH, Neel BG
Nature communications 2017 Sep 6;8(1):466
Nature communications 2017 Sep 6;8(1):466
Ageing causes prominent neurovascular dysfunction associated with loss of astrocytic contacts and gliosis.
Duncombe J, Lennen RJ, Jansen MA, Marshall I, Wardlaw JM, Horsburgh K
Neuropathology and applied neurobiology 2017 Oct;43(6):477-491
Neuropathology and applied neurobiology 2017 Oct;43(6):477-491
Disruption of Bmal1 Impairs Blood-Brain Barrier Integrity via Pericyte Dysfunction.
Nakazato R, Kawabe K, Yamada D, Ikeno S, Mieda M, Shimba S, Hinoi E, Yoneda Y, Takarada T
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Oct 18;37(42):10052-10062
The Journal of neuroscience : the official journal of the Society for Neuroscience 2017 Oct 18;37(42):10052-10062
Dual effects of carbon monoxide on pericytes and neurogenesis in traumatic brain injury.
Choi YK, Maki T, Mandeville ET, Koh SH, Hayakawa K, Arai K, Kim YM, Whalen MJ, Xing C, Wang X, Kim KW, Lo EH
Nature medicine 2016 Nov;22(11):1335-1341
Nature medicine 2016 Nov;22(11):1335-1341
Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration.
Chen WC, Wang Z, Missinato MA, Park DW, Long DW, Liu HJ, Zeng X, Yates NA, Kim K, Wang Y
Science advances 2016 Nov;2(11):e1600844
Science advances 2016 Nov;2(11):e1600844
VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination.
Miyamoto Y, Torii T, Tanoue A, Yamauchi J
Nature communications 2016 Nov 23;7:13478
Nature communications 2016 Nov 23;7:13478
Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation.
Frank DB, Peng T, Zepp JA, Snitow M, Vincent TL, Penkala IJ, Cui Z, Herriges MJ, Morley MP, Zhou S, Lu MM, Morrisey EE
Cell reports 2016 Nov 22;17(9):2312-2325
Cell reports 2016 Nov 22;17(9):2312-2325
Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke.
Szalay G, Martinecz B, Lénárt N, Környei Z, Orsolits B, Judák L, Császár E, Fekete R, West BL, Katona G, Rózsa B, Dénes Á
Nature communications 2016 May 3;7:11499
Nature communications 2016 May 3;7:11499
Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
Geng X, Cha B, Mahamud MR, Lim KC, Silasi-Mansat R, Uddin MKM, Miura N, Xia L, Simon AM, Engel JD, Chen H, Lupu F, Srinivasan RS
Developmental biology 2016 Jan 1;409(1):218-233
Developmental biology 2016 Jan 1;409(1):218-233
Nogo receptor blockade overcomes remyelination failure after white matter stroke and stimulates functional recovery in aged mice.
Sozmen EG, Rosenzweig S, Llorente IL, DiTullio DJ, Machnicki M, Vinters HV, Havton LA, Giger RJ, Hinman JD, Carmichael ST
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 27;113(52):E8453-E8462
Proceedings of the National Academy of Sciences of the United States of America 2016 Dec 27;113(52):E8453-E8462
Restoration of oligodendrocyte pools in a mouse model of chronic cerebral hypoperfusion.
McQueen J, Reimer MM, Holland PR, Manso Y, McLaughlin M, Fowler JH, Horsburgh K
PloS one 2014;9(2):e87227
PloS one 2014;9(2):e87227
Malignant stroma increases luminal breast cancer cell proliferation and angiogenesis through platelet-derived growth factor signaling.
Pinto MP, Dye WW, Jacobsen BM, Horwitz KB
BMC cancer 2014 Oct 1;14:735
BMC cancer 2014 Oct 1;14:735
Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility.
Cruz-Orengo L, Daniels BP, Dorsey D, Basak SA, Grajales-Reyes JG, McCandless EE, Piccio L, Schmidt RE, Cross AH, Crosby SD, Klein RS
The Journal of clinical investigation 2014 Jun;124(6):2571-84
The Journal of clinical investigation 2014 Jun;124(6):2571-84
Pericyte loss influences Alzheimer-like neurodegeneration in mice.
Sagare AP, Bell RD, Zhao Z, Ma Q, Winkler EA, Ramanathan A, Zlokovic BV
Nature communications 2013;4:2932
Nature communications 2013;4:2932
Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
The Journal of experimental medicine 2010 Apr 12;207(4):867-80
The Journal of experimental medicine 2010 Apr 12;207(4):867-80
Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice.
Ishii Y, Matsumoto Y, Watanabe R, Elmi M, Fujimori T, Nissen J, Cao Y, Nabeshima Y, Sasahara M, Funa K
Molecular and cellular neurosciences 2008 Mar;37(3):507-18
Molecular and cellular neurosciences 2008 Mar;37(3):507-18
Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 May 1;12(9):2676-88
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 May 1;12(9):2676-88
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Rat and Mouse PDGF R beta by Western Blot. Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- PDGF R beta in Mouse Embryonic Spinal Cord. PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
