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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-28527 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PDGFRB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-28527 targets PDGF Receptor beta in IF, IHC (P), and WB applications and shows reactivity with Human samples. The PA5-28527 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 201 of Human PDGF Receptor beta.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.63 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDGF-RB/CD140b using Rat tissue extracts (50 µg). Samples were loaded onto a 5% SDS-PAGE gel and probed with a PDGF-RB/CD140b polyclonal antibody (Product # PA5-28527) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PDGF-RB/CD140b using 30µg of A) HeLa S3 and B) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PDGF-RB/CD140b polyclonal antibody (Product # PA5-28527) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PDGF-RB/CD140b in methanol-fixed A431 cells using a PDGF-RB/CD140b polyclonal antibody (Product # PA5-28527) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PDGF-RB/CD140b in paraffin-embedded U87 xenograft using a PDGF-RB/CD140b polyclonal antibody (Product # PA5-28527) at a dilution of 1:500.
