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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX25511 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX25511, RRID:AB_380571
- Product name
- PDGF Receptor beta antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PDGFRB Antibody (N-term) (GTX25511) in cell line lysates (35μg/lane). PDGFRB (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PDGFRB Antibody (N-term)(GTX25511) with WiDr cell followed by Alexa Fluor 488-conjμgated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PDGFRB Antibody (N-term) (GTX25511), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SK-Br-3 cells using PDGFRB Antibody (N-term) (GTX25511) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
