AF1767

antibody from R&D Systems

Targeting: PDGFRB CD140b, JTK12, PDGFR, PDGFR1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1767

Provider

R&D Systems

Product name

Human Phospho-PDGF R beta (Y751) Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PDGF R beta when phosphorylated at Y751 in Western blots.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Vascular endothelial growth factor signals through platelet-derived growth factor receptor β in meningiomas in vitro.

Pfister C, Pfrommer H, Tatagiba MS, Roser F
British journal of cancer 2012 Nov 6;107(10):1702-13

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Phospho-PDGF R beta (Y751) by Western Blot. Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA, PDGF-AB, and PDGF-BB (Catalog # 221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Phospho-PDGF R beta (Y751) by Simple Western^TM. Simple Western lane view shows lysates of CCD-1070Sk human foreskin fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA (Catalog # 221-AA) for 10 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PDGF R beta (Y751) at approximately 274 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y751) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1767). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.