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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- FAB1263F - Provider product page
- Provider
- R&D Systems
- Product name
- Human PDGF R beta Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta-subunit complexed with 125I-PDGF-BB (3). It does not recognize the PDGF receptor alpha-subunit. Detects the PDGF receptor beta-subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- PR7212
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Somatic GNAQ Mutation is Enriched in Brain Endothelial Cells in Sturge-Weber Syndrome.
Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
Huang L, Couto JA, Pinto A, Alexandrescu S, Madsen JR, Greene AK, Sahin M, Bischoff J
Pediatric neurology 2017 Feb;67:59-63
Pediatric neurology 2017 Feb;67:59-63
Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells.
Huang L, Nakayama H, Klagsbrun M, Mulliken JB, Bischoff J
Stem cells (Dayton, Ohio) 2015 Jan;33(1):133-45
Stem cells (Dayton, Ohio) 2015 Jan;33(1):133-45
The axonal repellent Slit2 inhibits pericyte migration: potential implications in angiogenesis.
Guijarro-Muñoz I, Cuesta AM, Alvarez-Cienfuegos A, Geng JG, Alvarez-Vallina L, Sanz L
Experimental cell research 2012 Feb 15;318(4):371-8
Experimental cell research 2012 Feb 15;318(4):371-8
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of PDGF R beta in MG-63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1263F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
