16166-1-AP

antibody from Proteintech Group

Targeting: HDAC5 FLJ90614, KIAA0600, NY-CO-9

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16166-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16166-1-AP, RRID:AB_2116779

Product name

HDAC5-specific antibody

Antibody type

Polyclonal

Description

KD/KO validated HDAC5-specific antibody (Cat. #16166-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

African swine fever virus DEAD-box helicase D1133L promotes OGG1-driven incision of genomic 8-oxoG via HDAC5 deacetylation.

Fan J, Yang J, Tian Z, Zhang X, Geng S, Luo J, Boldogh I, Zeng Q, Yin H, Guan G, Niu Q
Journal of molecular cell biology 2026 Feb 13;17(7)

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NUPR1 contributes to endocrine therapy resistance by modulating BIRC5 expression and inducing luminal B-ERBB2(+) subtype-like characteristics in estrogen receptor-positive breast cancer cells.

Lee CH, Lin YC, Chang YC, Chen PC, Lin KH, Yeh TM, Leung EY, Lin IL, Chen SH, Cheung CHA
Journal of Cancer 2025;16(5):1694-1708

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Regulatory mechanism of ABCB1 transcriptional repression by HDAC5 in rat hepatocytes under hypoxic environment.

Wei Z, Mu H, Qiu F, Zhao M, Zhang X, Li W, Jia H, Wang R
Frontiers in physiology 2025;16:1520246

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Saikosaponin D Mitigates Radioresistance in Triple-Negative Breast Cancer by Inducing MRE11 De-Lactylation via HIF1α/HDAC5 Pathway.

Li J, Feng L, Zhang L, Zhang W, Zhang Y, Liu X, Cai Q, Zhao W, Huang G, Lu C
Theranostics 2025;15(17):8935-8951

---

Deacetylation of TALDO1 by HDAC6 promotes glycolysis and nasopharyngeal carcinoma progression through a moonlighting function.

Peng X, Zhou P, Zhang K, Chen L, Tang M, Zhou Q, Peng J, Yang L
Cell death & disease 2025 Oct 21;16(1):743

---

Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure.

Tong L, Wang Q, Zhang Y, Lai F, Xu J, Yin W, Zhang S, Wei G, Yin J, Yi H, Storm G, Wang Z, Huang R, Xu T, Wang JW
Biomaterials 2025 May;316:122976

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DHX9-mediated epigenetic silencing of BECN1 contributes to impaired autophagy and tumor progression in breast cancer via recruitment of HDAC5.

Li Z, Liu F, Li F, Zeng G, Wen X, Ding J, Zhou J
Cell death & disease 2025 Jul 14;16(1):524

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OGT mediated HDAC5 O-GlcNAcylation promotes osteogenesis by regulating the homeostasis of epigenetic modifications and proteolysis.

Du Y, Gao X, Chen J, Chen X, Liu H, He W, Liu L, Jiang Y, He B, Deng Z, Liang C, Guo F
Journal of orthopaedic translation 2025 Jan;50:14-29

---

Acetylation-enhanced Sp1 transcriptional activity suppresses Mlph expression.

Jo CS, Zhao H, Hwang JS
Scientific reports 2025 Jan 17;15(1):2338

---

Focal Adhesion Kinase Promotes Calcification of Vascular Smooth Muscle Cells via Regulation of Histone Deacetylase 4 and 5.

Tian W, Singh K, Kajuluri LP, Lu J, Lee S, Roh K, Nicholson CJ, Jiang W, Barnes HJ, Sato T, Boerboom S, Ostrom K, Li RH, Birchenough C, Moore E, Tattersfield H, Sigurslid HH, Mahamdeh MS, Mosquera JV, Hodonsky CJ, Johnson AL, Chou EL, Nigwekar S, Lindsay ME, Bloch DB, Lino Cardenas CL, Ichinose F, Miller CL, Wein MN, Malhotra R
Arteriosclerosis, thrombosis, and vascular biology 2025 Dec;45(12):e565-e583

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CK2B Induces CD8(+) T-Cell Exhaustion through HDAC8-Mediated Epigenetic Reprogramming to Limit the Efficacy of Anti-PD-1 Therapy in Non-Small-Cell Lung Cancer.

Liu S, Ma S, Liu G, Hou L, Guan Y, Liu L, Meng Y, Yu W, Liu T, Zhou L, Yuan Z, Pang S, Zhang S, Li J, Ren X, Sun Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(16):e2411053

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Osr2 functions as a biomechanical checkpoint to aggravate CD8(+) T cell exhaustion in tumor.

Zhang J, Li J, Hou Y, Lin Y, Zhao H, Shi Y, Chen K, Nian C, Tang J, Pan L, Xing Y, Gao H, Yang B, Song Z, Cheng Y, Liu Y, Sun M, Linghu Y, Li J, Huang H, Lai Z, Zhou Z, Li Z, Sun X, Chen Q, Su D, Li W, Peng Z, Liu P, Chen W, Huang H, Chen Y, Xiao B, Ye L, Chen L, Zhou D
Cell 2024 Jun 20;187(13):3409-3426.e24

---

GRK5 promoted renal fibrosis via HDAC5/Smad3 signaling pathway.

Xiang H, Huang J, Song A, Liu F, Xiong J, Zhang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Jan 31;38(2):e23422

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ATP citrate lyase drives vascular remodeling in systemic and pulmonary vascular diseases through metabolic and epigenetic changes.

Grobs Y, Romanet C, Lemay SE, Bourgeois A, Voisine P, Theberge C, Sauvaget M, Breuils-Bonnet S, Martineau S, El Kabbout R, Valasarajan C, Chelladurai P, Pelletier A, Mougin M, Dumais E, Perron J, Flamand N, Potus F, Provencher S, Pullamsetti SS, Boucherat O, Bonnet S
Science translational medicine 2024 Dec 11;16(777):eado7824

---

Down-regulation of histone deacetylase 7 reduces biological activities of retinal microvascular endothelial cells under high glucose condition and related mechanism.

Ning JY, Yang HY, Xie TK, Chen YX, Han J
International journal of ophthalmology 2023;16(8):1210-1217

---

HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma.

Sun X, Zhang K, Peng X, Zhou P, Qu C, Yang L, Shen L
Cancer letters 2023 May 28;562:216158

---

Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta.

Sun B, Wu H, Lu J, Zhang R, Shen X, Gu Y, Shi C, Zhang Y, Yuan W
Journal of orthopaedic translation 2023 Jan;38:175-189

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Selective Inhibition of Histone Deacetylase Class IIa With MC1568 Ameliorates Podocyte Injury.

He X, Sun T, Zhang P, Xia Z, Gao C, Ren H, Ji D
Frontiers in medicine 2022;9:848938

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HDAC5 inhibits ovarian angiogenesis in dehydroepiandrosterone-induced mouse model of polycystic ovary syndrome.

Wang Y, Wang Y, Chen Y, Gao Q, Hou L, Feng X
Folia histochemica et cytobiologica 2022;60(3):260-270

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ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer.

Zhou C, Li S, Bin K, Qin G, Pan P, Ren D, Zhao Y, Xia W, Chen J, Liu J, Wu H, Zhou Y
Cancer letters 2022 Oct 28;547:215855

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WNT5A promotes the metastasis of esophageal squamous cell carcinoma by activating the HDAC7/SNAIL signaling pathway.

Feng Y, Ma Z, Pan M, Xu L, Feng J, Zhang Y, Shao C, Guo K, Duan H, Zhang Y, Zhang Y, Zhang J, Lu D, Ren X, Han J, Li X, Yan X
Cell death & disease 2022 May 20;13(5):480

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microRNA-671-5p reduces tumorigenicity of ovarian cancer via suppressing HDAC5 and HIF-1α expression.

Peng D, Wu T, Wang J, Huang J, Zheng L, Wang P, Li J, Wu L, Luo M
Chemico-biological interactions 2022 Mar 1;355:109780

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EGCG prevents pressure overload‑induced myocardial remodeling by downregulating overexpression of HDAC5 in mice.

Han X, Peng C, Huang L, Luo X, Mao Q, Wu S, Zhang H
International journal of molecular medicine 2022 Jan;49(1)

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Development of a cancer cells self‑activating and miR‑125a‑5p expressing poly‑pharmacological nanodrug for cancer treatment.

Chang YC, Shieh MC, Chang YH, Huang WL, Su WC, Cheng FY, Cheung CHA
International journal of molecular medicine 2022 Aug;50(2)

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Trichostatin A increases BDNF protein expression by improving XBP-1s/ATF6/GRP78 axis in Schwann cells of diabetic peripheral neuropathy.

An J, Zhang X, Jia K, Zhang C, Zhu L, Cheng M, Li F, Zhao S, Hao J
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 Jan;133:111062

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Repeated stress exposure in mid-adolescence attenuates behavioral, noradrenergic, and epigenetic effects of trauma-like stress in early adult male rats.

Chaby LE, Sadik N, Burson NA, Lloyd S, O'Donnel K, Winters J, Conti AC, Liberzon I, Perrine SA
Scientific reports 2020 Oct 21;10(1):17935

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Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Feng S, Zhang L, Liu X, Li G, Zhang B, Wang Z, Zhang H, Ma H
Journal of cellular and molecular medicine 2020 Jul;24(14):7789-7801

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STAT3 phosphorylation mediates high glucose-impaired cell autophagy in an HDAC1-dependent and -independent manner in Schwann cells of diabetic peripheral neuropathy.

Du W, Wang N, Li F, Jia K, An J, Liu Y, Wang Y, Zhu L, Zhao S, Hao J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Jul;33(7):8008-8021

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Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Huang X, Yan J, Zhang M, Wang Y, Chen Y, Fu X, Wei R, Zheng XL, Liu Z, Zhang X, Yang H, Hao B, Shen YY, Su Y, Cong X, Huang M, Tan M, Ding J, Geng M
Cell 2018 Sep 20;175(1):186-199.e19

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HDAC2 and HDAC5 Up-Regulations Modulate Survivin and miR-125a-5p Expressions and Promote Hormone Therapy Resistance in Estrogen Receptor Positive Breast Cancer Cells.

Huang WT, Tsai YH, Chen SH, Kuo CW, Kuo YL, Lee KT, Chen WC, Wu PC, Chuang CY, Cheng SM, Lin CH, Leung EY, Chang YC, Cheung CHA
Frontiers in pharmacology 2017;8:902

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Antithetical regulation of α-myosin heavy chain between fetal and adult heart failure though shuttling of HDAC5 regulating YY-1 function.

Fang J, Li Y, Zhou K, Hua Y, Wang C, Mu D
Cardiovascular toxicology 2015 Apr;15(2):147-56

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Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Wang X, Liu J, Zhen J, Zhang C, Wan Q, Liu G, Wei X, Zhang Y, Wang Z, Han H, Xu H, Bao C, Song Z, Zhang X, Li N, Yi F
Kidney international 2014 Oct;86(4):712-25

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HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.

He M, Zhang B, Wei X, Wang Z, Fan B, Du P, Zhang Y, Jian W, Chen L, Wang L, Fang H, Li X, Wang PA, Yi F
Journal of cellular and molecular medicine 2013 Apr;17(4):531-42

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

human brain tissue were subjected to SDS PAGE followed by western blot with 16166-1-AP(HDAC5-specific antibody) at dilution of 1:300

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16166-1-AP(HDAC5-specific antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The HDAC5-specific antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human HDAC5-specific. This antibody recognizes human, mouse, rat antigen. The HDAC5-specific antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

16166-1-AP.pdf

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