PA5-37779

antibody from Invitrogen Antibodies

Targeting: HDAC5 FLJ90614, KIAA0600, NY-CO-9

Antibody data

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Validation data

Product number: PA5-37779 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-HDAC5 (Ser498) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: A suggested positive control for Western blot is 293 cells; suggested positive control for IHC is human breast carcinoma.
Concentration: 1 mg/mL

HDAC5 protein structure - PA5-37779 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of IMR32 (Lane 1), IMR32 treated with Ionomycin (0.5 uM for 1 min) (Lane 2), IMR32 treated with Ionomycin (0.5 uM for 5 min) (Lane 3), IMR32 treated with Ionomycin (0.5 uM for 60 min) (Lane 4), Jurkat (Lane 5), Jurkat treated with Ionomycin (0.5 uM for 1 min) (Lane 6), Jurkat treated with Ionomycin (0.5 uM for 5 min) (Lane 7), Jurkat treated with Ionomycin (0.5 uM for 60 min) (Lane 8). The blot was probed with Anti-Phospho-HDAC5 (Ser498) Polyclonal Antibody (Product # PA5-37779, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 122 kDa band corresponding to Phospho-HDAC5 (Ser259) was observed across the cell lines tested and showed treatment dependent difference in RAW 264.7 cell line upon Ionomycin treatment with varying time points.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of extracts from 293 cells, untreated or treated with serum starvation, using HDAC5 (pSer498) polyclonal antibody (Product # PA5-37779).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of IMR32 (Lane 1), IMR32 treated with Ionomycin (0.5 uM for 1 min) (Lane 2), IMR32 treated with Ionomycin (0.5 uM for 5 min) (Lane 3), IMR32 treated with Ionomycin (0.5 uM for 60 min) (Lane 4), Jurkat (Lane 5), Jurkat treated with Ionomycin (0.5 uM for 1 min) (Lane 6), Jurkat treated with Ionomycin (0.5 uM for 5 min) (Lane 7), Jurkat treated with Ionomycin (0.5 uM for 60 min) (Lane 8). The blot was probed with Anti-Phospho-HDAC5 (Ser498) Polyclonal Antibody (Product # PA5-37779, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 122 kDa band corresponding to Phospho-HDAC5 (Ser259) was observed across the cell lines tested and showed treatment dependent difference in RAW 264.7 cell line upon Ionomycin treatment with varying time points.