Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-37779 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-HDAC5 (Ser498) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is 293 cells; suggested positive control for IHC is human breast carcinoma. The homology between synthetic peptide sequence and rat is 100% .
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of IMR32 (Lane 1), IMR32 treated with Ionomycin (0.5 uM for 1 min) (Lane 2), IMR32 treated with Ionomycin (0.5 uM for 5 min) (Lane 3), IMR32 treated with Ionomycin (0.5 uM for 60 min) (Lane 4), Jurkat (Lane 5), Jurkat treated with Ionomycin (0.5 uM for 1 min) (Lane 6), Jurkat treated with Ionomycin (0.5 uM for 5 min) (Lane 7), Jurkat treated with Ionomycin (0.5 uM for 60 min) (Lane 8). The blot was probed with Anti-Phospho-HDAC5 (Ser498) Polyclonal Antibody (Product # PA5-37779, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 122 kDa band corresponding to Phospho-HDAC5 (Ser259) was observed across the cell lines tested and showed treatment dependent difference in RAW 264.7 cell line upon Ionomycin treatment with varying time points.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from 293 cells, untreated or treated with serum starvation, using HDAC5 (pSer498) polyclonal antibody (Product # PA5-37779).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of IMR32 (Lane 1), IMR32 treated with Ionomycin (0.5 uM for 1 min) (Lane 2), IMR32 treated with Ionomycin (0.5 uM for 5 min) (Lane 3), IMR32 treated with Ionomycin (0.5 uM for 60 min) (Lane 4), Jurkat (Lane 5), Jurkat treated with Ionomycin (0.5 uM for 1 min) (Lane 6), Jurkat treated with Ionomycin (0.5 uM for 5 min) (Lane 7), Jurkat treated with Ionomycin (0.5 uM for 60 min) (Lane 8). The blot was probed with Anti-Phospho-HDAC5 (Ser498) Polyclonal Antibody (Product # PA5-37779, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 122 kDa band corresponding to Phospho-HDAC5 (Ser259) was observed across the cell lines tested and showed treatment dependent difference in RAW 264.7 cell line upon Ionomycin treatment with varying time points.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-HDAC5 (Ser498) in paraffin-embedded Human breast carcinoma tissue using Phospho-HDAC5 (Ser498) Polyclonal Antibody (Product # PA5-37779) (left) or the same antibody preincubated with blocking peptide (right).