RQ4959

antibody from NSJ Bioreagents

Targeting: HDAC5 FLJ90614, KIAA0600, NY-CO-9

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4959

Provider

NSJ Bioreagents

Product name

HDAC5 Antibody

Antibody type

Polyclonal

Description

This highly specific HDAC5 antibody is suitable for use in Western blot/Immunohistochemistry/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the HDAC5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) placenta, 3) ThP-1, 4) K562, 5) PC-3 and 6) Caco-2 lysate with HDAC5 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa, observed at 122-160 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat heart, 2) rat liver, 3) rat stomach, 4) rat ovary, 5) mouse heart, 6) mouse liver, 7) mouse stomach, 8) mouse ovary and 9) mouse NIH3T3 lysate with HDAC5 antibody at 0.5ug/ml. Predicted molecular weight ~122 kDa, observed at 122-160 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse intestine with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat intestine with HDAC5 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.