Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- AP7630a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP7630a, RRID:AB_2099566
- Product name
- ERBB3 Antibody (N-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 1 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references High incidence of ErbB3, ErbB4, and MET expression in ovarian cancer.
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Neuregulin1-induced cell migration is impaired in schizophrenia: association with neuregulin1 and catechol-o-methyltransferase gene polymorphisms.
Davies S, Holmes A, Lomo L, Steinkamp MP, Kang H, Muller CY, Wilson BS
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2014 Jul;33(4):402-10
International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 2014 Jul;33(4):402-10
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
Circulation research 2009 Aug 28;105(5):500-10
Circulation research 2009 Aug 28;105(5):500-10
Neuregulin1-induced cell migration is impaired in schizophrenia: association with neuregulin1 and catechol-o-methyltransferase gene polymorphisms.
Sei Y, Ren-Patterson R, Li Z, Tunbridge EM, Egan MF, Kolachana BS, Weinberger DR
Molecular psychiatry 2007 Oct;12(10):946-57
Molecular psychiatry 2007 Oct;12(10):946-57
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Western blot analysis of anti-ErbB3 Pab (Cat. #AP7630a) in mouse brain lysate. ErbB3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:10~50
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human breast carcinoma with ErbB3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."
- Primary Ab dilution
- 1:50~100
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Flow cytometric analysis of 293 cells using ErbB3 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50