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- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [2]
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- Product number
- MA5-13053 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB3 Monoclonal Antibody (2C3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-13053 targets HER-3 in IP and WB applications and shows reactivity with Human, mouse, and Rat samples. The MA5-13053 immunogen is recombinant rat c-erbB-3/HER-3 oncoprotein.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2C3
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references The Hippo Pathway Transducers YAP1/TEAD Induce Acquired Resistance to Trastuzumab in HER2-Positive Breast Cancer.
Functional cooperation of miR-125a, miR-125b, and miR-205 in entinostat-induced downregulation of erbB2/erbB3 and apoptosis in breast cancer cells.
Estrogenic promotion of ErbB2 tyrosine kinase activity in mammary tumor cells requires activation of ErbB3 signaling.
A physical mechanism for coupling bone resorption and formation in adult human bone.
Src and ADAM-17-mediated shedding of transforming growth factor-alpha is a mechanism of acute resistance to TRAIL.
Species differences in renal Src activity direct EGF receptor regulation in life or death response to EGF.
Breast tumor kinase (protein tyrosine kinase 6) regulates heregulin-induced activation of ERK5 and p38 MAP kinases in breast cancer cells.
Downregulation of erbB3 abrogates erbB2-mediated tamoxifen resistance in breast cancer cells.
Muc4-ErbB2 complex formation and signaling in polarized CACO-2 epithelial cells indicate that Muc4 acts as an unorthodox ligand for ErbB2.
H-RAS V12-induced radioresistance in HCT116 colon carcinoma cells is heregulin dependent.
A new superinvasive in vitro phenotype induced by selection of human breast carcinoma cells with the chemotherapeutic drugs paclitaxel and doxorubicin.
ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes.
Roles of mitogen-activated protein kinase and phosphoinositide 3'-kinase in ErbB2/ErbB3 coreceptor-mediated heregulin signaling.
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
Radiosensitization of malignant glioma cells through overexpression of dominant-negative epidermal growth factor receptor.
ErbB3 (HER3) interaction with the p85 regulatory subunit of phosphoinositide 3-kinase.
González-Alonso P, Zazo S, Martín-Aparicio E, Luque M, Chamizo C, Sanz-Álvarez M, Minguez P, Gómez-López G, Cristóbal I, Caramés C, García-Foncillas J, Eroles P, Lluch A, Arpí O, Rovira A, Albanell J, Piersma SR, Jimenez CR, Madoz-Gúrpide J, Rojo F
Cancers 2020 Apr 29;12(5)
Cancers 2020 Apr 29;12(5)
Functional cooperation of miR-125a, miR-125b, and miR-205 in entinostat-induced downregulation of erbB2/erbB3 and apoptosis in breast cancer cells.
Wang S, Huang J, Lyu H, Lee CK, Tan J, Wang J, Liu B
Cell death & disease 2013 Mar 21;4(3):e556
Cell death & disease 2013 Mar 21;4(3):e556
Estrogenic promotion of ErbB2 tyrosine kinase activity in mammary tumor cells requires activation of ErbB3 signaling.
Liu B, Ordonez-Ercan D, Fan Z, Huang X, Edgerton SM, Yang X, Thor AD
Molecular cancer research : MCR 2009 Nov;7(11):1882-92
Molecular cancer research : MCR 2009 Nov;7(11):1882-92
A physical mechanism for coupling bone resorption and formation in adult human bone.
Andersen TL, Sondergaard TE, Skorzynska KE, Dagnaes-Hansen F, Plesner TL, Hauge EM, Plesner T, Delaisse JM
The American journal of pathology 2009 Jan;174(1):239-47
The American journal of pathology 2009 Jan;174(1):239-47
Src and ADAM-17-mediated shedding of transforming growth factor-alpha is a mechanism of acute resistance to TRAIL.
Van Schaeybroeck S, Kelly DM, Kyula J, Stokesberry S, Fennell DA, Johnston PG, Longley DB
Cancer research 2008 Oct 15;68(20):8312-21
Cancer research 2008 Oct 15;68(20):8312-21
Species differences in renal Src activity direct EGF receptor regulation in life or death response to EGF.
Kiley SC, Chevalier RL
American journal of physiology. Renal physiology 2007 Sep;293(3):F895-903
American journal of physiology. Renal physiology 2007 Sep;293(3):F895-903
Breast tumor kinase (protein tyrosine kinase 6) regulates heregulin-induced activation of ERK5 and p38 MAP kinases in breast cancer cells.
Ostrander JH, Daniel AR, Lofgren K, Kleer CG, Lange CA
Cancer research 2007 May 1;67(9):4199-209
Cancer research 2007 May 1;67(9):4199-209
Downregulation of erbB3 abrogates erbB2-mediated tamoxifen resistance in breast cancer cells.
Liu B, Ordonez-Ercan D, Fan Z, Edgerton SM, Yang X, Thor AD
International journal of cancer 2007 May 1;120(9):1874-82
International journal of cancer 2007 May 1;120(9):1874-82
Muc4-ErbB2 complex formation and signaling in polarized CACO-2 epithelial cells indicate that Muc4 acts as an unorthodox ligand for ErbB2.
Ramsauer VP, Pino V, Farooq A, Carothers Carraway CA, Salas PJ, Carraway KL
Molecular biology of the cell 2006 Jul;17(7):2931-41
Molecular biology of the cell 2006 Jul;17(7):2931-41
H-RAS V12-induced radioresistance in HCT116 colon carcinoma cells is heregulin dependent.
Carón RW, Yacoub A, Zhu X, Mitchell C, Han SI, Sasazuki T, Shirasawa S, Hagan MP, Grant S, Dent P
Molecular cancer therapeutics 2005 Feb;4(2):243-55
Molecular cancer therapeutics 2005 Feb;4(2):243-55
A new superinvasive in vitro phenotype induced by selection of human breast carcinoma cells with the chemotherapeutic drugs paclitaxel and doxorubicin.
Glynn SA, Gammell P, Heenan M, O'Connor R, Liang Y, Keenan J, Clynes M
British journal of cancer 2004 Nov 15;91(10):1800-7
British journal of cancer 2004 Nov 15;91(10):1800-7
ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes.
Pagano E, Calvo JC
Journal of cellular biochemistry 2003 Oct 15;90(3):561-72
Journal of cellular biochemistry 2003 Oct 15;90(3):561-72
Roles of mitogen-activated protein kinase and phosphoinositide 3'-kinase in ErbB2/ErbB3 coreceptor-mediated heregulin signaling.
Vijapurkar U, Kim MS, Koland JG
Experimental cell research 2003 Apr 1;284(2):291-302
Experimental cell research 2003 Apr 1;284(2):291-302
Reciprocal signaling between spiral ganglion neurons and Schwann cells involves neuregulin and neurotrophins.
Hansen MR, Vijapurkar U, Koland JG, Green SH
Hearing research 2001 Nov;161(1-2):87-98
Hearing research 2001 Nov;161(1-2):87-98
Radiosensitization of malignant glioma cells through overexpression of dominant-negative epidermal growth factor receptor.
Lammering G, Valerie K, Lin PS, Mikkelsen RB, Contessa JN, Feden JP, Farnsworth J, Dent P, Schmidt-Ullrich RK
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Mar;7(3):682-90
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Mar;7(3):682-90
ErbB3 (HER3) interaction with the p85 regulatory subunit of phosphoinositide 3-kinase.
Hellyer NJ, Cheng K, Koland JG
The Biochemical journal 1998 Aug 1;333 ( Pt 3)(Pt 3):757-63
The Biochemical journal 1998 Aug 1;333 ( Pt 3)(Pt 3):757-63
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of HER-3 using HER-3 Monoclonal Antibody (Product # MA5-13053) on SKBR3 Cells and MAD109 Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HER-3 was done on A-375 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with HER-3 Mouse Monoclonal Antibody (MA513053, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of HER-3 using HER-3 Monoclonal Antibody (Product # MA5-13053) on denatured Human T47D Cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 ( A ) The generation of trastuzumab resistance induced changes in the abundance and phosphorylation patterns of HER family members in BT-474.r2T cell line. Trastuzumab-sensitive and -resistant BT-474 cells were compared by WB for their protein levels at basal no-treatment conditions. beta-actin was used as a loading control. ( B ) Changes were observed for different markers of EMT. ( C ) The acquisition of resistance to trastuzumab enhances transwell migration in resistant cells as compared to parental, sensitive BT-474 cells (* denotes p < 0.05).
