LS-C148233

antibody from LSBio

Targeting: BCL2L1 Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C148233

Provider

LSBio

Product name

BCL2L1 / BCL-XL Antibody (aa46-66) LS-C148233

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Human, Mouse, Rat, Canine, Hamster, Porcine

Host

Rabbit

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot of Bcl-X in lysates from isolated rat liver mitochondria using Polyclonal Antibody to Bcl-XL+S at 1:2000. The antibody detected Bcl-XL at ~30 kD.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Analysis of Bcl-X in human breast carcinoma using Polyclonal Antibody to Bcl-XL+S at 1:2000. A. Formalin-fixed, paraffin-embedded tissue section from invasive ductal carcinoma. An organellar/mitochondrial pattern of Bcl-X staining is observed in the cytosol of tumor cells. B. Western blot of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kD) is is detected. Lane 8. Recombinant Bcl-XL (positive control).

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-X expression using Polyclonal Antibody to Bcl-XL+S. Visualized with DAB (A) or VIP (B) chromogens. Hematoxylin-Eosin counterstain.