Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- MAB894-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Bcl-xL Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human Bcl-xL in direct ELISAs and detects human, mouse, and rat Bcl-xL in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
Q07817
- Isotype
- IgG
- Antibody clone number
- 2052A
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The novel TRAIL-receptor agonist APG350 exerts superior therapeutic activity in pancreatic cancer cells.
Legler K, Hauser C, Egberts JH, Willms A, Heneweer C, Boretius S, Röcken C, Glüer CC, Becker T, Kluge M, Hill O, Gieffers C, Fricke H, Kalthoff H, Lemke J, Trauzold A
Cell death & disease 2018 May 1;9(5):445
Cell death & disease 2018 May 1;9(5):445
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Bcl-xL by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, Jurkat human acute T cell leukemia cell line, C6 rat glioma cell line, and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl-xL Monoclonal Antibody (Catalog # MAB894) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-xL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Bcl-xL by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl-xL at approximately 33 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Bcl-xL Monoclonal Antibody (Catalog # MAB894) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.