R32762

antibody from NSJ Bioreagents

Targeting: BCL2L1 Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R32762

Provider

NSJ Bioreagents

Product name

Bcl-XL Antibody

Antibody type

Polyclonal

Description

This highly specific Bcl-XL antibody is suitable for use in Western blot/Flow cytometry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Bcl-XL antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat thymus, 2) mouse NIH3T3, 3) mouse Hepa1-6, 4) human SGC-7901 and 5) human HepG2 lysate with Bcl-XL antibody at 0.5ug/ml. Expected molecular weight: 26-30 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human Jurkat, 2) human HeLa, 3) human A549, 4) human Raji, 5) rat brain, 6) rat C6, 7) mouse brain and 8) mouse NIH 3T3 cell lysate with Bcl-XL antibody at 0.5ug/ml. Expected molecular weight: 26-30 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast cancer tissue with Bcl-XL antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human tonsil tissue with Bcl-XL antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung cancer tissue with Bcl-XL antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human Jurkat cells with Bcl-XL antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bcl-XL antibody.