MAB5860

antibody from Abnova Corporation

Targeting: BCL2L1 Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5860

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB5860, RRID:AB_10549906

Product name

BCL2L1 monoclonal antibody, clone 7B2.5 (Biotin)

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against recombinant BCL2L1.

Conjugate

Biotin

Isotype

IgG

Antibody clone number

7B2.5

Storage

Store in the dark at 4°C. Do not freeze.Avoid prolonged exposure to light.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Bax-independent inhibition of apoptosis by Bcl-XL.

Cheng EH, Levine B, Boise LH, Thompson CB, Hardwick JM
Nature 1996 Feb 8;379(6565):554-6

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Prevention of hypoxia-induced cell death by Bcl-2 and Bcl-xL.

Shimizu S, Eguchi Y, Kosaka H, Kamiike W, Matsuda H, Tsujimoto Y
Nature 1995 Apr 27;374(6525):811-3

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Modulation of apoptosis by the widely distributed Bcl-2 homologue Bak.

Kiefer MC, Brauer MJ, Powers VC, Wu JJ, Umansky SR, Tomei LD, Barr PJ
Nature 1995 Apr 20;374(6524):736-9

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Murine FL5 cells (FL5-NEO) and FL5 cells transfected with Bcl-xL expression plasmid (FL5-BCL-XL) were fixed with buffered paraformaldehyde and then permeabilized with saponin. The cells were incubated with BCL2L1 monoclonal antibody, clone 7B2.5 followed by phycoerythrin-conjugated goat anti-mouse IgG, and then were analyzed by flow cytometry.

Validation comment

Flow Cytometry