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LearnMA5-14011
antibody from Invitrogen Antibodies
Targeting: BCL2L1
Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-14011 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bcl-X Monoclonal Antibody (2H12)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. The immunogenic sequence (aa 3-14) is conserved in human and mice. Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. A recommended positive control is Hodgkin's lymphoma.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H12
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Molecular prognosticators of complex karyotype soft tissue sarcoma outcome: a tissue microarray-based study.
Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells.
Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells.
Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.
Minocycline up-regulates Bcl-2 and protects against cell death in mitochondria.
Hypoxia selection of death-resistant cells. A role for Bcl-X(L).
Bcl-x(S) induces an NGF-inhibitable cytochrome c release.
Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.
Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+ circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation.
Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival.
Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival.
Lahat G, Tuvin D, Wei C, Wang WL, Pollock RE, Anaya DA, Bekele BN, Corely L, Lazar AJ, Pisters PW, Lev D
Annals of oncology : official journal of the European Society for Medical Oncology 2010 May;21(5):1112-20
Annals of oncology : official journal of the European Society for Medical Oncology 2010 May;21(5):1112-20
Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells.
Schulze-Bergkamen H, Ehrenberg R, Hickmann L, Vick B, Urbanik T, Schimanski CC, Berger MR, Schad A, Weber A, Heeger S, Galle PR, Moehler M
World journal of gastroenterology 2008 Jun 28;14(24):3829-40
World journal of gastroenterology 2008 Jun 28;14(24):3829-40
Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells.
Schulze-Bergkamen H, Ehrenberg R, Hickmann L, Vick B, Urbanik T, Schimanski CC, Berger MR, Schad A, Weber A, Heeger S, Galle PR, Moehler M
World journal of gastroenterology 2008 Jun 28;14(24):3829-40
World journal of gastroenterology 2008 Jun 28;14(24):3829-40
Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.
Shaknovich R, Basso K, Bhagat G, Mansukhani M, Hatzivassiliou G, Murty VV, Buettner M, Niedobitek G, Alobeid B, Cattoretti G
Haematologica 2006 Oct;91(10):1313-20
Haematologica 2006 Oct;91(10):1313-20
Minocycline up-regulates Bcl-2 and protects against cell death in mitochondria.
Wang J, Wei Q, Wang CY, Hill WD, Hess DC, Dong Z
The Journal of biological chemistry 2004 May 7;279(19):19948-54
The Journal of biological chemistry 2004 May 7;279(19):19948-54
Hypoxia selection of death-resistant cells. A role for Bcl-X(L).
Dong Z, Wang J
The Journal of biological chemistry 2004 Mar 5;279(10):9215-21
The Journal of biological chemistry 2004 Mar 5;279(10):9215-21
Bcl-x(S) induces an NGF-inhibitable cytochrome c release.
Lindenboim L, Schlipf S, Kaufmann T, Borner C, Stein R
Experimental cell research 2004 Jul 15;297(2):392-403
Experimental cell research 2004 Jul 15;297(2):392-403
Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.
Sharif M, Whitehouse A, Sharman P, Perry M, Adams M
Arthritis and rheumatism 2004 Feb;50(2):507-15
Arthritis and rheumatism 2004 Feb;50(2):507-15
Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+ circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation.
Ferrero E, Belloni D, Contini P, Foglieni C, Ferrero ME, Fabbri M, Poggi A, Zocchi MR
Blood 2003 Jan 1;101(1):186-93
Blood 2003 Jan 1;101(1):186-93
Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival.
Korkolopoulou P, Lazaris ACh, Konstantinidou AE, Kavantzas N, Patsouris E, Christodoulou P, Thomas-Tsagli E, Davaris P
European urology 2002 Mar;41(3):274-83
European urology 2002 Mar;41(3):274-83
Differential expression of bcl-2 family proteins in bladder carcinomas. Relationship with apoptotic rate and survival.
Korkolopoulou P, Lazaris ACh, Konstantinidou AE, Kavantzas N, Patsouris E, Christodoulou P, Thomas-Tsagli E, Davaris P
European urology 2002 Mar;41(3):274-83
European urology 2002 Mar;41(3):274-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCL-X in CEM cell extracts using a BCL-X monoclonal antibody (Product # MA5-14011).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human Hodgkins lymphoma stained with Bcl-X antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of Reedternberg cells.
