Ab090843
antibody from Aladdin Scientific
Targeting: BCL2L1
Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- Ab090843 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Bcl-XL Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Bcl-XL Antibody, Recombinant, could be used for WB, IHC, IF, ICC and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein FunctionPotent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.Isoform Bcl-X(S) promotes apoptosis.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-210
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Bcl-XL Antibody (Ab090843) - Western Blot All lanes: Recombinant Bcl-XL Antibody (Ab090843) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution Predicted band size: 26 kDa Observed band size: 28 kDa Exposure time: 10.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Bcl-XL Antibody (Ab090843) - IF/ICC Immunocytochemistry analysis of Bcl-XL (green) in HT-1080 using Recombinant Bcl-XL Antibody (Ab090843), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Bcl-XL Antibody (Ab090843) - IHC Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Recombinant Bcl-XL Antibody (Ab090843). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.