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LearnMA5-14008
antibody from Invitrogen Antibodies
Targeting: BCL2L1
Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-14008 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Bcl-X Monoclonal Antibody (2H12), Biotin
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins. The immunogenic sequence (aa 3-14) is conserved in human and mice. Staining of formalin-fixed paraffin-embedded tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at room temperature for 20 min. A recommended positive control is Hodgkin's lymphoma.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 2H12
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references Bcl-x(L) and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells.
Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.
Minocycline up-regulates Bcl-2 and protects against cell death in mitochondria.
Hypoxia selection of death-resistant cells. A role for Bcl-X(L).
Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.
Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+ circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation.
Schulze-Bergkamen H, Ehrenberg R, Hickmann L, Vick B, Urbanik T, Schimanski CC, Berger MR, Schad A, Weber A, Heeger S, Galle PR, Moehler M
World journal of gastroenterology 2008 Jun 28;14(24):3829-40
World journal of gastroenterology 2008 Jun 28;14(24):3829-40
Identification of rare Epstein-Barr virus infected memory B cells and plasma cells in non-monomorphic post-transplant lymphoproliferative disorders and the signature of viral signaling.
Shaknovich R, Basso K, Bhagat G, Mansukhani M, Hatzivassiliou G, Murty VV, Buettner M, Niedobitek G, Alobeid B, Cattoretti G
Haematologica 2006 Oct;91(10):1313-20
Haematologica 2006 Oct;91(10):1313-20
Minocycline up-regulates Bcl-2 and protects against cell death in mitochondria.
Wang J, Wei Q, Wang CY, Hill WD, Hess DC, Dong Z
The Journal of biological chemistry 2004 May 7;279(19):19948-54
The Journal of biological chemistry 2004 May 7;279(19):19948-54
Hypoxia selection of death-resistant cells. A role for Bcl-X(L).
Dong Z, Wang J
The Journal of biological chemistry 2004 Mar 5;279(10):9215-21
The Journal of biological chemistry 2004 Mar 5;279(10):9215-21
Increased apoptosis in human osteoarthritic cartilage corresponds to reduced cell density and expression of caspase-3.
Sharif M, Whitehouse A, Sharman P, Perry M, Adams M
Arthritis and rheumatism 2004 Feb;50(2):507-15
Arthritis and rheumatism 2004 Feb;50(2):507-15
Transendothelial migration leads to protection from starvation-induced apoptosis in CD34+CD14+ circulating precursors: evidence for PECAM-1 involvement through Akt/PKB activation.
Ferrero E, Belloni D, Contini P, Foglieni C, Ferrero ME, Fabbri M, Poggi A, Zocchi MR
Blood 2003 Jan 1;101(1):186-93
Blood 2003 Jan 1;101(1):186-93
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human Hodgkins lymphoma stained with Bcl-X antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of Reedternberg cells.
- Conjugate
- Biotin
