Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- AF800 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Bcl-x Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse Bcl-x in Western blots.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint.
Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
Schmitt E, Beauchemin M, Bertrand R
Oncogene 2007 Aug 30;26(40):5851-65
Oncogene 2007 Aug 30;26(40):5851-65
Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.
Armstrong T, Packham G, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Nov 1;10(21):7427-37
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Nov 1;10(21):7427-37
Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8
Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.
Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8
The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.
Sade H, Krishna S, Sarin A
The Journal of biological chemistry 2004 Jan 23;279(4):2937-44
The Journal of biological chemistry 2004 Jan 23;279(4):2937-44
Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
Raman VS, Akondy RS, Rath S, Bal V, George A
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81
Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.
Raman VS, Akondy RS, Rath S, Bal V, George A
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81
T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
The Journal of experimental medicine 2002 Jul 1;196(1):87-95
The Journal of experimental medicine 2002 Jul 1;196(1):87-95
T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.
Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
The Journal of experimental medicine 2002 Jul 1;196(1):87-95
The Journal of experimental medicine 2002 Jul 1;196(1):87-95
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Bcl-x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 104 cells, lane 1 and 2.5 x 104 cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Bcl-x by Simple WesternTM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl-x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Immunoprecipitation of Human Bcl-x. Bcl-x was immunoprecipitated from lysates (7 x 105 cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30-60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.