609-401-D12
antibody from Rockland Immunochemicals, Inc.
Targeting: CTNNB1
armadillo, beta-catenin, CTNNB
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 609-401-D12 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#609-401-D12, RRID:AB_11182904
- Product name
- Anti-beta Catenin pS33/pS37 (Rabbit) Antibody - 609-401-D12
- Antibody type
- Polyclonal
- Description
- Anti-Beta Catenin pS33/pS37 Antibody is directed against beta human Catenin protein phosphorylated ar S33/pS37. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated beta Catenin. Minimal reactivity occurs against non-phosphorylated beta Catenin. Reactivity against beta Catenin occurs from human sources. However, reactivity is also expected against mouse, rat and Xenopus based on 100% sequence homology. Cross reactivity from other sources has not been determined.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- Titrated value sufficient to run approximately 10 mini blots. *
- Storage
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
- Handling
- Dry Ice
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-beta Catenin pS33/pS37 antibody. Lane 1: human embryonic kidney cell (HEK) lysate. Lane 2: human embryonic kidney cell (HEK) lysate with glycogen synthase kinase 3? knocked down by SiRNA. Load: 10 µg per lane. Upper Panel: ?-catenin phosphorylated at Ser33 and Ser37. Lower Panel: pan ?-catenin. Primary antibody: ?-catenin pS33 and S37 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~83 kDa, ~83 kDa for ?-catenin pS33 and S37. Other band(s): none.
- Validation comment
- Western Blot