Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6308 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Collagen IV alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Collagen IV alpha 1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse COL13A1, recombinant human (rh) COL25A1 and rhCOL23A1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Gelatinolytic activity of autocrine matrix metalloproteinase-9 leads to endothelial de-arrangement in Moyamoya disease.
Blecharz-Lang KG, Prinz V, Burek M, Frey D, Schenkel T, Krug SM, Fromm M, Vajkoczy P
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2018 Nov;38(11):1940-1953
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 2018 Nov;38(11):1940-1953
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Collagen IV alpha 1 by Western Blot. Western blot shows lysates of human placenta tissue. PVDF Membrane was probed with 1 µg/mL of Human Collagen IV alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6308) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen IV a1 at approximately 95 kDa (as indicated) ref 1. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.